publication . Article . 2015

The metabolism of 4-bromoaniline in the bile-cannulated rat: application of ICPMS (79/81Br), HPLC-ICPMS & HPLC-oaTOFMS

Duckett, Catherine; McCullagh, Michael; Smith, Christopher; Wilson, Ian D;
Open Access English
  • Published: 01 Aug 2015 Journal: Xenobiotica; the Fate of Foreign Compounds in Biological Systems, volume 45, issue 8, pages 672-680 (issn: 0049-8254, eissn: 1366-5928, Copyright policy)
  • Publisher: Informa Healthcare
Abstract
© 2015 The Author(s). Published by Taylor & Francis.1. An excretion balance study was performed following i.p. administration of 4-bromoaniline (50 mg kg<sup>-1</sup>) to bile-cannulated rats, using bromine-detected (<sup>79/81</sup>Br) ICPMS for quantification. Approximately 90% of the dose was recovered in urine (68.9 ± 3.6%) and bile (21.4 ± 1.4%) by 48 h post-administration.2. HPLC-ICPMS (<sup>79/81</sup>Br) was used to selectively detect and profile the major urinary and biliary-excreted metabolites and determined that the 0-12 h urine contained at least 21 brominated metabolites with 19 bromine-containing peaks observed in the 6-12 h bile samples.3. The ur...
Subjects
free text keywords: HPLC-ICPMS, HPLC-MS, Aniline metabolism, bromine detection, Animal Pharmacokinetics and Metabolism, bromine-based excretion balance study
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publication . Article . 2015

The metabolism of 4-bromoaniline in the bile-cannulated rat: application of ICPMS (79/81Br), HPLC-ICPMS & HPLC-oaTOFMS

Duckett, Catherine; McCullagh, Michael; Smith, Christopher; Wilson, Ian D;