Expression and purification of the non-tagged LipL32 of pathogenic Leptospira

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Hauk,P.; Carvalho,E.; Ho,P.L.;
(2011)
  • Publisher: Associação Brasileira de Divulgação Científica
  • Journal: Brazilian Journal of Medical and Biological Research (issn: 0100-879X, eissn: 1414-431X)
  • Publisher copyright policies & self-archiving
  • Related identifiers: doi: 10.1590/S0100-879X2011007500025
  • Subject: Vaccine | R5-920 | Medicine (General) | Diagnosis | Antigen | Non-tagged protein purification | Biology (General) | LipL32 | Pathogenic Leptospira | QH301-705.5

Leptospirosis is a reemerging infectious disease and the most disseminated zoonosis worldwide. A leptospiral surface protein, LipL32, only occurs in pathogenic Leptospira, and is the most abundant protein on the bacterial surface, being described as an important factor ... View more
  • References (20)
    20 references, page 1 of 2

    1. Adler B, de la Pena MA. Leptospira and leptospirosis. Vet Microbiol 2010; 140: 287-296.

    2. Malmstrom J, Beck M, Schmidt A, Lange V, Deutsch EW, Aebersold R. Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans. Nature 2009; 460: 762-765.

    3. Haake DA, Chao G, Zuerner RL, Barnett JK, Barnett D, Mazel M, et al. The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection. Infect Immun 2000; 68: 2276-2285.

    4. Picardeau M, Bulach DM, Bouchier C, Zuerner RL, Zidane N, Wilson PJ, et al. Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis. PLoS One 2008; 3: e1607.

    5. Yang CW, Wu MS, Pan MJ, Hsieh WJ, Vandewalle A, Huang CC. The Leptospira outer membrane protein LipL32 induces tubulointerstitial nephritis-mediated gene expression in mouse proximal tubule cells. J Am Soc Nephrol 2002; 13: 2037-2045.

    6. Palaniappan RU, Ramanujam S, Chang YF. Leptospirosis: pathogenesis, immunity, and diagnosis. Curr Opin Infect Dis 2007; 20: 284-292.

    7. Hauk P, Macedo F, Romero EC, Vasconcellos SA, de Morais ZM, Barbosa AS, et al. In LipL32, the major leptospiral lipoprotein, the C terminus is the primary immunogenic domain and mediates interaction with collagen IV and plasma ifbronectin. Infect Immun 2008; 76: 2642-2650.

    8. Hoke DE, Egan S, Cullen PA, Adler B. LipL32 is an extracellular matrix-interacting protein of Leptospira spp and Pseudoalteromonas tunicata. Infect Immun 2008; 76: 2063-2069.

    9. Murray GL, Srikram A, Hoke DE, Wunder EA Jr, Henry R, Lo M, et al. Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans. Infect Immun 2009; 77: 952-958.

    10. Bhandari P, Gowrishankar J. An Escherichia coli host strain useful for efficient overproduction of cloned gene products with NaCl as the inducer. J Bacteriol 1997; 179: 4403- 4406.

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