publication . Article . 2015

Decoupled illumination detection in light sheet microscopy for fast volumetric imaging

Olarte, Omar; Andilla, Jordi; Artigas García, David; Loza-Alvarez, Pablo;
Open Access English
  • Published: 04 Aug 2015
  • Publisher: OSA
Abstract
Current microscopy demands the visualization of large three-dimensional samples with increased sensitivity, higher resolution, and faster speed. Several imaging techniques based on widefield, point-scanning, and light-sheet strategies have been designed to tackle some of these demands. Although successful, all these require the illuminated volumes to be tightly coupled with the detection optics to accomplish efficient optical sectioning. Here, we break this paradigm and produce optical sections from out-of-focus planes. This is done by extending the depth of field of the detection optics in a light-sheet microscope using wavefront-coding techniques. This passive...
Subjects
free text keywords: :Enginyeria de la telecomunicació::Telecomunicació òptica::Fotònica [Àrees temàtiques de la UPC], Fotònica, Imaging systems, :Física [Àrees temàtiques de la UPC], Visió, imaging, Photonics
Funded by
EC| LASERLAB-EUROPE
Project
LASERLAB-EUROPE
The Integrated Initiative of European Laser Research Infrastructures III
  • Funder: European Commission (EC)
  • Project Code: 284464
  • Funding stream: FP7 | SP4 | INFRA
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publication . Article . 2015

Decoupled illumination detection in light sheet microscopy for fast volumetric imaging

Olarte, Omar; Andilla, Jordi; Artigas García, David; Loza-Alvarez, Pablo;