Multiplexed Single Intact Cell Droplet Digital PCR (MuSIC ddPCR) Method for Specific Detection of Enterohemorrhagic E. coli (EHEC) in Food Enrichment Cultures

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McMahon, Tanis C.; Blais, Burton W.; Wong, Alex; Carrillo, Catherine D.;
(2017)
  • Publisher: Frontiers Media S.A.
  • Journal: Frontiers in Microbiology,volume 8 (issn: 1664-302X, eissn: 1664-302X)
  • Related identifiers: doi: 10.3389/fmicb.2017.00332, pmc: PMC5332415
  • Subject: Technology Report | quantitative PCR | droplet digital PCR | Microbiology | STEC | Shiga toxin | EHEC | intimin

Foodborne illness attributed to enterohemorrhagic E. coli (EHEC), a highly pathogenic subset of Shiga toxin-producing E. coli (STEC), is increasingly recognized as a significant public health issue. Current microbiological methods for identification of EHEC in foods oft... View more
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