Enantiocomplementary Yarrowia lipolytica Oxidoreductases: Alcohol Dehydrogenase 2 and Short Chain Dehydrogenase/Reductase

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Napora-Wijata, Kamila; Strohmeier, Gernot; Sonavane, Manoj; Avi, Manuela; Robins, Karen; Winkler, Margit;
  • Publisher: Multidisciplinary Digital Publishing Institute
  • Journal: Biomolecules,volume 3,issue 3,pages449-460 (issn: 2218-273X, eissn: 2218-273X)
  • Related identifiers: doi: 10.3390/biom3030449, pmc: PMC4030946
  • Subject: medium chain secondary alcohols | Yarrowia lipolytica | <i>Yarrowia lipolytica</i> | biooxidation | Microbiology | QR1-502 | Zn-dependent alcohol dehydrogenase | enantioselective reduction | Article | short chain dehydrogenase/reductase

Enzymes of the non-conventional yeast Yarrowia lipolytica seem to be tailor-made for the conversion of lipophilic substrates. Herein, we cloned and overexpressed the Zn-dependent alcohol dehydrogenase ADH2 from Yarrowia lipolytica in Escherichia coli. The purified enzym... View more
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