Detection of Potato Leaf Roll Virus (PLRV), Potato Virus Y (PVY) and Potato Virus X (PVX) on Five Potato Varieties by Using of DAS-ELISA and RT-PCR Methods
PLRV, PVY, PVX, DAS-ELISA, RT-PCR [Keywords] | PLRV, PVY, PVX, DAS-ELISA, RT-PCR
mesheuropmc: food and beverages | fungi
Potato is a staple food crop that widely grown around the world. Virus infection is main factor that affects great loss of the potato production. Potato virus X(PVX), potato virus Y(PVY),and potato leaf roll virus(PLRV) are top three viruses that result in decreased yield of potato in Indonesia. Therefore, the rapid methods of DAS-ELISA was studied to test tuber samples of five potato varieties, Granola, Atlantik, Raja, Super John, Kalosi, and Masalle. Two simple, rapid, sensitive, reliable detection techniques of three virus are reported in the paper: they are DAS-ELISA and RT-PCR. Detection of all three viruses on positive control samples could be carried out with ELISA technique without purification of RNA virus. Neither PLRV nor PVX were detected on five potato varieties test samples, but PVY was detected only in Kalosi test sample. This method is simpler and rapider than RT-PCR technique, and is more suitable for the detection of a great number of potato samples. The sensitivity of ELISA is as same as that of RT-PCR.
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