publication . Article . 2009

The amoebal MAP kinase response to Legionella pneumophila is regulated by DupA

Li, Zhiru; Dugan, Aisling S.; Bloomfield, Gareth; Skelton, Jason; Ivens, Alasdair; Losick, Vicki; Isberg, Ralph R.;
Open Access English
  • Published: 17 Sep 2009 Journal: volume 6, issue 3, pages 253-267issn: 1931-3128, Copyright policy
  • Publisher: Elsevier BV
Abstract
The amoeba Dictyostelium discoideum can support replication of Legionella pneumophila. Here we identify the dupA gene, encoding a putative tyrosine kinase/dual-specificity phosphatase, in a screen for D. discoideum mutants altered in allowing L. pneumophila intracellular replication. Inactivation of dupA resulted in depressed L. pneumophila growth and sustained hyperphosphorylation of the amoebal MAP kinase ERK1, consistent with loss of a phosphatase activity. Bacterial challenge of wild-type amoebae induced dupA expression and resulted in transiently increased ERK1 phosphorylation, suggesting that dupA and ERK1 are part of a response to bacteria. Indeed, over 5...
Subjects
mesheuropmc: bacteriarespiratory tract diseasesmacromolecular substances
free text keywords: Molecular Biology, Protein-Tyrosine Kinases, Immunology and Microbiology(all), Protozoan Proteins, Cancer Research, Legionella pneumophila, Gene Expression Regulation, Dictyostelium, Host-Pathogen Interactions, /dk/atira/pure/subjectarea/asjc/2400, Phosphoric Monoester Hydrolases, /dk/atira/pure/subjectarea/asjc/1300/1312, /dk/atira/pure/subjectarea/asjc/1300/1306, Animals, Mitogen-Activated Protein Kinase 3, Phosphorylation
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publication . Article . 2009

The amoebal MAP kinase response to Legionella pneumophila is regulated by DupA

Li, Zhiru; Dugan, Aisling S.; Bloomfield, Gareth; Skelton, Jason; Ivens, Alasdair; Losick, Vicki; Isberg, Ralph R.;