Expression of IMP1 Enhances Production of Murine Leukemia Virus Vector by Facilitating Viral Genomic RNA Packaging

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Mai, Yun ; Gao, Guangxia (2010)
  • Publisher: Public Library of Science
  • Journal: PLoS ONE, volume 5, issue 12 (issn: 1932-6203, eissn: 1932-6203)
  • Related identifiers: pmc: PMC3012120, doi: 10.1371/journal.pone.0015881
  • Subject: Human Genetics | Research Article | Biology | Viruslike Particles | Infectious Diseases | Microbiology | Gene Therapy | Medicine | Viral Vectors | Viral Diseases | Viral Replication | Q | R | Genetics | HIV | Virology | Vector Biology | Science | Viral Packaging | Viral Nucleic Acid
    mesheuropmc: viruses

Murine leukemia virus (MLV)-based retroviral vector is widely used for gene transfer. Efficient packaging of the genomic RNA is critical for production of high-titer virus. Here, we report that expression of the insulin-like growth factor II mRNA binding protein 1 (IMP1) enhanced the production of infectious MLV vector. Overexpression of IMP1 increased the stability of viral genomic RNA in virus producer cells and packaging of the RNA into progeny virus in a dose-dependent manner. Downregulation of IMP1 in virus producer cells resulted in reduced production of the retroviral vector. These results indicate that IMP1 plays a role in regulating the packaging of MLV genomic RNA and can be used for improving production of retroviral vectors.
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