publication . Article . Other literature type . 2016

Raman activated cell sorting

Song, Yizhi; Yin, Huabing; Huang, Wei E.;
Open Access
  • Published: 01 Aug 2016 Journal: Current Opinion in Chemical Biology, volume 33, pages 1-8 (issn: 1367-5931, Copyright policy)
  • Publisher: Elsevier BV
  • Country: United Kingdom
Single cell Raman spectra (SCRS) are intrinsic biochemical profiles and ‘chemical images’ of single cells which can be used to characterise phenotypic changes, physiological states and functions of cells. On the base of SCRS, Raman activated cell sorting (RACS) provides a label-free cell sorting approach, which can link single cells to their chemical or phenotypic profiles. Overcoming naturally weak Raman signals, establishing Raman biomarker as sorting criteria to RACS and improving specific sorting technology are three challenges of developing RACS. Advances on Raman spectroscopy such as stimulated Raman scattering (SRS) and pre-screening helped to increase RA...
Medical Subject Headings: inorganic chemicalsmacromolecular substances
free text keywords: Analytical Chemistry, Biochemistry
Related Organizations
66 references, page 1 of 5

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2. Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow, G12 8QQ, UK.

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** Excellent and elegant work developing a universal C-D Raman biomarker using D2O, and employing Raman tweezers to sort metabolically active cells in a complex microbial community.

66 references, page 1 of 5
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