publication . Article . Other literature type . 2018

Sub-Ensemble Monitoring of DNA Strand Displacement Using Multiparameter Single-Molecule FRET.

Steven W. Magennis; Laura E. Baltierra-Jasso; Michael J. Morten;
Open Access
  • Published: 05 Mar 2018 Journal: ChemPhysChem, volume 19, pages 551-555 (issn: 1439-4235, Copyright policy)
  • Publisher: Wiley
  • Country: United Kingdom
Abstract
Non-enzymatic DNA strand displacement is an important mechanism in dynamic DNA nanotechnology. Here, we show that the large parameter space that is accessible by single-molecule FRET is ideal for the simultaneous monitoring of multiple reactants and products of DNA strand exchange reactions. We monitored the strand displacement from double-stranded DNA (dsDNA) by single-stranded DNA (ssDNA) at 37 °C; the data were modelled as a second-order reaction approaching equilibrium, with a rate constant of 10 m-1  s-1 . We also followed the displacement from a DNA three-way junction (3WJ) by ssDNA. The presence of three internal mismatched bases in the middle of the inva...
Subjects
free text keywords: Physical and Theoretical Chemistry, Atomic and Molecular Physics, and Optics, DNA nanotechnology, Duplex (building), Förster resonance energy transfer, Chemistry, Biophysics, Fluorescence, Single-molecule FRET, A-DNA, DNA, chemistry.chemical_compound, Reaction rate constant
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