High throughput multiplex real time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

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Otti, Gerald ; Bouvaine, Sophie ; Kimata, Bernadetha ; Mkamillo, Geoffrey ; Kumar, Lava ; Tomlins, Keith ; Maruthi, M.N. (2016)
  • Publisher: Blackwell Publishing
  • Related identifiers: doi: 10.1111/jam.13043
  • Subject:
    mesheuropmc: food and beverages | viruses

Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa.\ud \ud Methods and Results: The diagnostic assay was developed for... View more
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