A“Proteoglycan Targeting Strategy” for the\ud Scintigraphic Imaging and Monitoring of the Swarm Rat\ud Chondrosarcoma Orthotopic Model

Article English OPEN
Peyrode, C. ; Gouin, F. ; Vidal, A. ; Auzeloux, P. ; Besse, S. ; Dauplat, M.M. ; Askienazy, S. ; Heymann, D. ; Chezal, J.M. ; Redini, F. ; Miot-Noirault, E. (2011)
  • Publisher: Hindawi Publishing Corporation
  • Subject:
    mesheuropmc: viruses

Our lab developed 99mTc-NTP 15-5 radiotracer as targeting proteoglycans (PGs) for the scintigraphic imaging of joint. This paper\ud reports preclinical results of 99mTc-NTP 15-5 imaging of an orthotopic model of Swarm rat chondrosarcoma (SRC). 99mTc-NTP\ud 15-5 imaging of SRC-bearing and sham-operated animals was performed and quantified at regular intervals after surgery and\ud compared to bone scintigraphy and tumoural volume. Tumours were characterized by histology and PG assay. SRC exhibited\ud a significant 99mTc-NTP 15-5 uptake at very early stage after implant (with tumour/muscle ratio of 1.61 ± 0.14), whereas no\ud measurable tumour was evidenced. As tumour grew, mean tumour/muscle ratio was increased by 2.4, between the early and late\ud stage of pathology. Bone scintigraphy failed to image chondrosarcoma, even at the later stage of study. 99mTc-NTP 15-5 imaging\ud provided a suitable set of quantitative criteria for the in vivo characterization of chondrosarcoma behaviour in bone environment,\ud useful for achieving a greater understanding of the pathology.
  • References (35)
    35 references, page 1 of 4

    [1] J. C. Madelmont, I. Giraud, C. Nicolas et al., “inventors. Cyclopharma Laboratoires, assignee. Novel quaternary ammonium derivatives, method for preparing same and pharmaceutical use,” International patent PCT WO 01/00621 A1. January 2001.

    [2] C. Nicolas, M. Borel, J. C. Maurizis et al., “Synthesis of Nquaternary ammonium [3H] and [99mTc]Polyazamacrocycles, potential radiotracers for cartilage imaging,” Journal of Labelled Compounds and Radiopharmaceuticals, vol. 43, no. 6, pp. 585-594, 2000.

    [3] J. C. Maurizis, M. Rapp, C. Nicolas, M. Ollier, M. Verny, and J. C. Madelmont, “Disposition in rats of N-pyridiniumpropyl-cyclam, N- triethylammoniumpropyl-cyclam, and N[triethylammonium]-3-propyl-[15]ane-N5, potential cartilage imaging agents,” Drug Metabolism and Disposition, vol. 28, no. 4, pp. 418-422, 2000.

    [4] M. Ollier, J. C. Maurizis, C. Nicolas et al., “Joint scintigraphy in rabbits with 99mTc-N-[3-(triethylammonio)propyl]-15aneN5, a new radiodiagnostic agent for articular cartilage imaging,” Journal of Nuclear Medicine, vol. 42, no. 1, pp. 141-145, 2001.

    [5] E. Miot-Noirault, A. Vidal, P. Pastoureau et al., “Early detection and monitoring of cartilage alteration in the experimental meniscectomised guinea pig model of osteoarthritis by 99mTcNTP 15-5 scintigraphy,” European Journal of Nuclear Medicine and Molecular Imaging, vol. 34, no. 8, pp. 1280-1290, 2007.

    [6] E. Miot-Noirault, A. Vidal, P. Auzeloux, J. C. Madelmont, J. Maublant, and N. Moins, “First in vivo SPECT imaging of mouse femorotibial cartilage using 99mTc-NTP 15-5,” Molecular Imaging, vol. 7, no. 6, pp. 263-271, 2008.

    [7] F. Lamoureux, M. Baud'huin, L. Duplomb, D. Heymann, and F. Re´dini, “Proteoglycans: key partners in bone cell biology,” BioEssays, vol. 29, no. 8, pp. 758-771, 2007.

    [8] World Health Organization, “Cartilage tumours,” in World Health Organization Classification of Tumours: Pathology and Genetics, Tumours of Soft Tissue and Bone, C. D. M. Fletcher, K. K. Unni, and F. Mertens, Eds., pp. 234-257, IARC Press, Lyon, France, 2002.

    [9] H. Gelderblom, P. C. W. Hogendoorn, S. D. Dijkstra et al., “The clinical approach towards chondrosarcoma,” Oncologist, vol. 13, no. 3, pp. 320-329, 2008.

    [10] W. A. Chow, “Update on chondrosarcomas,” Current Opinion in Oncology, vol. 19, no. 4, pp. 371-376, 2007.

  • Similar Research Results (4)
  • Metrics
    0
    views in OpenAIRE
    0
    views in local repository
    44
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    White Rose Research Online - IRUS-UK 0 44
Share - Bookmark