publication . Article . Other literature type . 2017

A reductive aminase from Aspergillus oryzae.

Aleku, Godwin A.; France, Scott P.; Man, Henry; Mangas-Sanchez, Juan; Montgomery, Sarah L.; Sharma, Mahima; Leipold, Friedemann; Hussain, Shahed; Grogan, Gideon; Turner, Nicholas J.;
  • Published: 29 May 2017
Abstract
Reductive amination is one of the most important methods for the synthesis of chiral amines. Here we report the discovery of an NADP(H)-dependent reductive aminase from Aspergillus oryzae (AspRedAm, Uniprot code Q2TW47) that can catalyse the reductive coupling of a broad set of carbonyl compounds with a variety of primary and secondary amines with up to >98% conversion and with up to >98% enantiomeric excess. In cases where both carbonyl and amine show high reactivity, it is possible to employ a 1:1 ratio of the substrates, forming amine products with up to 94% conversion. Steady-state kinetic studies establish that the enzyme is capable of catalysing imin...
Subjects
free text keywords: General Chemistry, General Chemical Engineering, Aspergillus oryzae, biology.organism_classification, biology, Reductive amination, Organic chemistry, Biocatalysis, Catalysis, Imine, chemistry.chemical_compound, chemistry, Oxidoreductase, chemistry.chemical_classification, Mutagenesis, Enzyme, 1600, 1500

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Abstract
Reductive amination is one of the most important methods for the synthesis of chiral amines. Here we report the discovery of an NADP(H)-dependent reductive aminase from Aspergillus oryzae (AspRedAm, Uniprot code Q2TW47) that can catalyse the reductive coupling of a broad set of carbonyl compounds with a variety of primary and secondary amines with up to >98% conversion and with up to >98% enantiomeric excess. In cases where both carbonyl and amine show high reactivity, it is possible to employ a 1:1 ratio of the substrates, forming amine products with up to 94% conversion. Steady-state kinetic studies establish that the enzyme is capable of catalysing imin...
Subjects
free text keywords: General Chemistry, General Chemical Engineering, Aspergillus oryzae, biology.organism_classification, biology, Reductive amination, Organic chemistry, Biocatalysis, Catalysis, Imine, chemistry.chemical_compound, chemistry, Oxidoreductase, chemistry.chemical_classification, Mutagenesis, Enzyme, 1600, 1500

10 11 12 13 14 15 16 17 18 19 20 21 22 23 AspRedAm

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