Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein

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Reddington, Samuel C.; Driezis, Sarunas; Hartley, Andrew M.; Watson, Peter Duncan; Rizkallah, Pierre J.; Jones, Darran Dafydd;

The photochemical properties of phenyl azide have been exploited to modulate the function of a red autofluorescent protein, mCherry. Using genetic code reprogramming, phenyl azide chemistry has been introduced at functionally strategic positions in mCherry leading to de... View more
  • References (21)
    21 references, page 1 of 3

    1 K. Deisseroth, Nat. Methods, 2011, 8, 26-29.

    2 A. Gautier, C. Gauron, M. Volovitch, D. Bensimon, L. Jullien and S. Vriz, Nat. Chem. Biol., 2014, 10, 533-541.

    3 J. E. Toettcher, C. A. Voigt, O. D. Weiner and W. A. Lim, Nat. Methods, 2011, 8, 35-38.

    4 A. Moglich and K. Moffat, Photochem. Photobiol. Sci., 2010, 9, 1286-1300.

    5 A. Gautier, A. Deiters and J. W. Chin, J. Am. Chem. Soc., 2011, 133, 2124-2127.

    6 J. Chin, S. Santoro, A. Martin, D. King, L. Wang and P. Schultz, J. Am. Chem. Soc., 2002, 124, 9026-9027.

    7 S. Reddington, P. Watson, P. Rizkallah, E. Tippmann and D. D. Jones, Biochem. Soc. Trans., 2013, 41, 1177-1182.

    8 J. L. Morris, S. C. Reddington, D. M. Murphy, D. D. Jones, J. A. Platts and E. M. Tippmann, Org. Lett., 2013, 15, 728-731.

    9 A. Miyawaki, D. M. Shcherbakova and V. V. Verkhusha, Curr. Opin. Struct. Biol., 2012, 22, 679-688.

    10 R. Y. Tsien, Annu. Rev. Biochem., 1998, 67, 509-544.

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