The effects of storage conditions on viability of Clostridium difficile vegetative cells and spores and toxin activity in human faeces\ud
Freeman, J.; Wilcox, M.H.;
AIMS: Clostridium difficile is a common nosocomial pathogen and as such diagnostic and research\ud methods may necessitate storage of faecal specimens for long periods, followed by subsequent\ud re-examination. This study investigated the effects of storage conditions u... View more
1 Brazier JS. Role of the laboratory in investigations of Clostridium difficile diarrhoea. Clin Infect Dis 1993;4(suppl):S228-33.
2 Bowman RA, Riley TV. Isolation of Clostridium difficile from stored specimens and comparative susceptibility of various tissue culture cell lines to cytotoxin. FEMS Microbiol Lett 1986;34:31-5.
3 Borriello SP, Vale T, Brazier JS, et al. Evaluation of a commercial enzyme immunoassay for the detection of Clostridium difficile toxin A. Eur J Clin Microbiol Infect Dis 1988;7:476-84.
4 Weese JS, Staempfli HR, Prescott JF. Survival of Clostridium difficile and its toxins in equine feces: implications for diagnostic test selection and interpretation. J Vet Diagn Invest 2000;12:332-6.
5 Wilcox MH, Fawley WN, Parnell P. Value of lysozyme agar incorporation and alkaline thioglycollate exposure for the environmental recovery of Clostridium difficile. J Hosp Infect 2000;44:65-9.
6 Borriello SP, Honour P. Simplified procedure for the routine isolation of Clostridium difficile from faeces. J Clin Pathol 1981;34:1124-7.
7 May T, Mackie RI, Fahey GC, Jr, et al. Effect of fiber source on short-chain fatty acid production and on the growth and toxin production by Clostridium difficile. Scand J Gastroenterol 1994;29:916-22.
8 Ito Y, Moriwaki H, Muto Y, Kato N, et al. Effect of lactulose on short-chain fatty acids and lactate production and on the growth of faecal flora, with special reference to Clostridium difficile. J Med Microbiol 1997;46:80-4.
9 Futter BV, Richardson G. Viability of Clostridial spores and requirements of damaged organisms. I Method of colony count, period and temperature of incubation and pH value of the medium. J Appl Bacteriol 1970;33:321-30.
10 Brazier JS. The epidemiology and typing of Clostridium difficile. J Antimicrob Chemother 1998;41(suppl 4):C47-57.