publication . Article . 1998

Localization of the Functional Domains of Human Tissue Inhibitor of Metalloproteinases-3 and the Effects of a Sorsby's Fundus Dystrophy Mutation \ud

Langton, K.P.; Barker, M.D.; McKie, N.;
Open Access English
  • Published: 03 Jul 1998
  • Country: United Kingdom
Abstract
A transient COS-7 cell expression system was used to\ud investigate the functional domain arrangement of tissue\ud inhibitor of metalloproteinases-3 (TIMP-3), specifically\ud to assess the contribution of the amino- and carboxylterminal\ud domains of the molecule to its matrix metalloproteinase\ud (MMP) inhibitory and extracellular matrix\ud (ECM) binding properties. Wild type TIMP-3 was entirely\ud localized to the ECM in both its glycosylated (27\ud kDa) and unglycosylated (24 kDa) forms. A COOH-terminally\ud truncated TIMP-3 molecule was found to be a non-\ud ECM bound MMP inhibitor, whereas a chimeric TIMP\ud molecule, consisting of the NH2-terminal domain o...
38 references, page 1 of 3

1. Docherty, A. J. P., Lyons, A., Smith, B. J., Wright, E. M., Stephens, P. E., Harris, T. J. R., Murphy, G., and Reynolds, J. J. (1985) Nature 318, 66 - 69

2. Boone, T. C., Johnson, M. J., Declerck, Y. A., and Langley, K. E. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 2800 -2804

3. Apte, S. S., Mattei, M. G., and Olsen, B. R. (1994) Genomics 19, 86 -90

4. Greene, J., Wang, M., Liu, Y. E., Raymond, L. A., Rosen, C., and Shi, Y. E. (1996) J. Biol. Chem. 271, 30375-30380

5. Murphy, G., and Hembry, R. M. (1992) J. Rheumatol. 19, (suppl.) 61- 64

6. Celentano, D. C., and Frishman, W. H. (1997) J. Clin. Pharmacol. 37, 991-1000

7. Stetler-Stevenson, W. G., Hewitt, R., and Corcoran, M. (1996) Semin. Cancer Biol. 7, 147-154

8. Murphy, G., and Willenbrock, F. (1995) Methods Enzymol. 248, 496 -510

9. Goldberg, G. I., Strongin, A., Collier, I. E., Genrich, L. T., and Marmer, B. L. (1992) J. Biol. Chem. 267, 4583- 4591

10. Howard, E. W., Bullen, E. C., and Banda, M. J. (1991) J. Biol. Chem. 266, 13064 -13069

11. Bigg, H. F., Shi, Y. E., Liu, Y. E., Steffensen, B., and Overall, C. M. (1997) J. Biol. Chem. 272, 15496 -15500

12. Ogata, Y., Itoh, Y., and Nagase, H. (1995) J. Biol. Chem. 270, 18506 -18511

13. Thorgeirsson, U. P., Yoshiji, H., Sinha, C. C., and Gomez, D. E. (1996) In Vivo 10, 137-144

14. Chesler, L., Golde, D. W., Bersch, N., and Johnson, M. D. (1995) Blood 86, 4506 - 4515

15. Murphy, A. N., Unsworth, E. J., and Stetler-Stevenson, W. G. (1993) J. Cell. Physiol. 157, 351-358

38 references, page 1 of 3
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