publication . Article . 1998

Localization of the Functional Domains of Human Tissue Inhibitor of Metalloproteinases-3 and the Effects of a Sorsby's Fundus Dystrophy Mutation \ud

Langton, K.P.; Barker, M.D.; McKie, N.;
Open Access English
  • Published: 03 Jul 1998
  • Country: United Kingdom
A transient COS-7 cell expression system was used to\ud investigate the functional domain arrangement of tissue\ud inhibitor of metalloproteinases-3 (TIMP-3), specifically\ud to assess the contribution of the amino- and carboxylterminal\ud domains of the molecule to its matrix metalloproteinase\ud (MMP) inhibitory and extracellular matrix\ud (ECM) binding properties. Wild type TIMP-3 was entirely\ud localized to the ECM in both its glycosylated (27\ud kDa) and unglycosylated (24 kDa) forms. A COOH-terminally\ud truncated TIMP-3 molecule was found to be a non-\ud ECM bound MMP inhibitor, whereas a chimeric TIMP\ud molecule, consisting of the NH2-terminal domain o...
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