Stable isotope techniques for the assessment of host and microbiota response during gastrointestinal dysfunction

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Butler, Ross N. ; Kosek, Margaret ; Krebs, Nancy F. ; Loechl, Cornelia ; Loy, Alexander ; Owino, Vcitor ; Zimmermann, Michael B. ; Morrison, Douglas J. (2017)

The International Atomic Energy Agency convened a technical meeting on environmental enteric dysfunction (EED) in Vienna (28th – 30th October 2015; to bring together international experts in the fields of EED, nutrition and stable isotope technologies. Advances in stable isotope labelling techniques open up new possibilities to improve our understanding of gastrointestinal dysfunction and the role of the microbiota in host health. In the context of EED, little is known about the role gut dysfunction may play in macro- and micronutrient bioavailability and requirements and what the consequences may be for nutritional status and linear growth. Stable isotope labelling techniques have been used to assess intestinal mucosal injury and barrier function, carbohydrate digestion and fermentation, protein derived amino acid bioavailability and requirements, micronutrient bioavailability and to track microbe-microbe and microbe-host interactions at the single cell level. The non-invasive nature of stable isotope technologies potentially allows for low-hazard, field deployable tests of gut dysfunction that are applicable across all age-groups. The purpose of this review is to assess the state-of-the-art in the use of stable isotope technologies and to provide a perspective on where these technologies can be exploited to further our understanding of gut dysfunction in EED.
  • References (69)
    69 references, page 1 of 7

    1. Kosek M, Guerrant RL, Kang G, et al. Assessment of environmental enteropathy in the MAL-ED cohort study: theoretical and analytic framework. Clin Infect Dis 2014;59(suppl 4):S239-47.

    2. Campbell DI, McPhail G, Lunn PG, et al. Intestinal inflammation measured by fecal neopterin in Gambian children with enteropathy: association with growth failure, Giardia lamblia, and intestinal permeability. J Pediatr Gastroenterol Nutr 2004;39:153-7.

    3. Black RE, Victora CG, Walker SP, et al. Maternal and child undernutrition and overweight in low-income and middle-income countries. Lancet 2013;382:427-51.

    4. Bhutta ZA, Das JK, Rizvi A, et al. Evidence-based interventions for improvement of maternal and child nutrition: what can be done and at what cost? Lancet 2013;382:452-77.

    5. Trehan I, Kelly P, Shaikh N, et al. New insights into environmental enteric dysfunction. Arch Dis Child 2016;101:741-4.

    6. Keusch GT, Denno DM, Black RE, et al. Environmental enteric dysfunction: pathogenesis, diagnosis, and clinical consequences. Clin Infect Dis 2014;59(suppl 4):S207-12.

    7. Romagnuolo J, Schiller D, Bailey RJ. Using breath tests wisely in a gastroenterology practice: an evidence-based review of indications and pitfalls in interpretation. Am J Gastroenterol 2002;97:1113-26.

    8. Klein PD, Klein ER. Applications of stable isotopes to pediatric nutrition and gastroenterology: measurement of nutrient absorption and digestion using 13C. J Pediatr Gastroenterol Nutr 1985;4:9-19.

    9. Leal YA, Flores LL, Fuentes-Panana EM, et al. 13C-urea breath test for the diagnosis of Helicobacter pylori infection in children: a systematic review and meta-analysis. Helicobacter 2011;16:327-37.

    10. Dror G, Muhsen K. Helicobacter pylori infection and children's growth: an overview. J Pediatr Gastroenterol Nutr 2016;62:e48-59.

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