Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1

Article English OPEN
Carr, SB ; Mecia, LB ; Phillips, SEV ; Thomas, CD (2013)
  • Publisher: International Union of Crystallography
  • Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, volume 69, issue Pt 10, pages 1,123-1,126 (eissn: 1744-3091)
  • Related identifiers: pmc: PMC3792671, doi: 10.1107/S1744309113023828
  • Subject: plasmid pSTK1 | plasmid pT181 | replication initiation proteins | Crystallization Communications | Geobacillus stearothermophilus | rolling-circle replication | Staphylococcus aureus

Antibiotic resistance in bacterial pathogens poses an ever-increasing risk to human health. In antibiotic-resistant strains of Staphylococcus aureus this resistance often resides in extra-chromosomal plasmids, such as those of the pT181 family, which replicate via a rolling-circle mechanism mediated by a plasmid-encoded replication initiation protein. Currently, there is no structural information available for the pT181-family Rep proteins. Here, the crystallization of a catalytically active fragment of a homologous replication initiation protein from the thermophile Geobacillus stearothermophilus responsible for the replication of plasmid pSTK1 is reported. Crystals of the RepSTK1 fragment diffracted to a resolution of 2.5 Å and belonged to space group P212121.
  • References (23)
    23 references, page 1 of 3

    Battye, T. G. G., Kontogiannis, L., Johnson, O., Powell, H. R. & Leslie, A. G. W. (2011). Acta Cryst. D67, 271-281.

    Boer, D. R., Ru´ız-Mas o´, J. A., Lo´ pez-Blanco, J. R., Blanco, A. G., Vives-Ll a`cer, M., Chaco´ n, P., Uso´ n, I., Gomis-Ru¨ th, F. X., Espinosa, M., Llorca, O., del Solar, G. & Collect, M. (2009). EMBO J. 28, 1666-1678.

    Boer, R., Russi, S., Guasch, A., Lucas, M., Blanco, A. G., P e´rez-Luque, R., Coll, M. & de la Cruz, F. (2006). J. Mol. Biol. 358, 857-869.

    Caryl, J. A., Smith, M. C. & Thomas, C. D. (2004). J. Bacteriol. 186, 3374-3383.

    Evans, P. R. (2011). Acta Cryst. D67, 282-292.

    Hickman, A. B., Ronning, D. R., Kotin, R. M. & Dyda, F. (2002). Mol. Cell, 10, 327-337.

    Klimenko, D. E., Convery, M. A., Rowsell, S., Thomas, C. D. & Phillips, S. E. V. (1999). Acta Cryst. D55, 1076-1078.

    Koepsel, R. R., Murray, R. W., Rosenblum, W. D. & Khan, S. A. (1985). Proc. Natl Acad. Sci. USA, 82, 6845-6849.

    Koonin, E. V. & Ilyina, T. V. (1993). Biosystems, 30, 241-268.

    Lee, C. A. & Grossman, A. D. (2007). J. Bacteriol. 189, 7254-7261.

  • Metrics
    No metrics available