Enzymatic Digestion and Selective Quantification of Underivatised Delta-9-Tetrahydrocannabinol and Cocaine in Human Hair Using Gas Chromatography-Mass Spectrometry

Article English OPEN
Breidi, Salah Eddine ; Barker, James ; Petróczi, Andrea ; Naughton, Declan P. (2012)
  • Publisher: Hindawi Publishing Corporation
  • Journal: Journal of Analytical Methods in Chemistry, volume 2,012 (issn: 2090-8865, eissn: 2090-8873)
  • Related identifiers: doi: 10.1155/2012/907893, pmc: PMC3335444
  • Subject: alliedhealth | Research Article | Article Subject
    mesheuropmc: mental disorders

Gas chromatography-mass spectrometric (GC-MS) methods for drug analysis routinely employ derivatising reagents. The aim of this paper was to develop a method for the analysis of two recreational drugs, delta-9-tetrahydrocannabinol ([delta](9)-THC) and cocaine in hair samples using GC-MS, without prior derivatisation, thus allowing the sample to be reanalysed in its original form. An enzymatic digestion technique was also developed. Ten hair samples, that were known positive for either [delta](9)-THC and/or cocaine, were enzymatically digested, extracted, and then analysed by GC-MS. All samples measured contained [delta](9)-THC and one sample contained cocaine. The limits of detection (LOD) and quantification (LOQ) were 0.02 ng/mg and 0.05 ng/mg, respectively, for cocaine and 0.015 ng/mg and 0.02 ng/mg, respectively, for [delta](9)-THC. The wide detection window, ease of direct analysis by GC-MS, lower detection limits of underivatised samples, and the stability of drugs using this technique may offer an improved method of analysis.
  • References (32)
    32 references, page 1 of 4

    [1] United Nation Office on Drugs and Crimes (UNODC), world drug report, 2011, http://www.unodc.org/documents/dataand-analysis/WDR2011-ExSum.pdf.

    [2] World Health Organization (WHO), Management of substance abuse (Other psychoactive substances), 2010, http:// www.who.int/substance abuse/facts/psychoactives/en/index .html.

    [3] T. Nadulski, S. Bleeck, J. Schra¨der, W. R. Bork, and F. Pragst, “11-Nor-Δ9-tetrahydrocannabinol-9-carboxylic acid ethyl ester (THC-COOEt): unsuccessful search for a marker of combined cannabis and alcohol consumption,” Forensic Science International, vol. 196, no. 1-3, pp. 78-84, 2010.

    [4] G. Kauert and J. Ro¨hrich, “Concentrations of Δ9-tetrahydrocannabinol, cocaine and 6-monoacetylmorphine in hair of drug abusers,” International Journal of Legal Medicine, vol. 108, no. 6, pp. 294-299, 1996.

    [5] N. D. Campbell, “Suspect technologies: scrutinizing the intersection of science, technology, and policy,” Science Technology and Human Values, vol. 30, no. 3, pp. 374-402, 2005.

    [6] R. Kronstrand, I. Nystro¨m, M. Forsman, and K. Ka¨ll, “Hair analysis for drugs in driver's license regranting. A Swedish pilot study,” Forensic Science International, vol. 196, no. 1-3, pp. 55-58, 2010.

    [7] M. C. Ricossa, M. Bernini, and F. De Ferrari, “Hair analysis for driving licence in cocaine and heroin users. An epidemiological study,” Forensic Science International, vol. 107, no. 1-3, pp. 301-308, 2000.

    [8] M. J. Hannett, “Lessening the sting of ASFA: the rehabilitation-relapse dilemma brought about by drug addiction and termination of parental rights,” Family Court Review, vol. 45, pp. 345-353, 2007.

    [9] R. Agius and P. Kintz, “Guidelines for European workplace drug and alcohol testing in hair,” Drug Testing and Analysis, vol. 2, no. 8, pp. 367-376, 2010.

    [10] L. Tsanaclis and J. F. C. Wicks, “Patterns in drug use in the United Kingdom as revealed through analysis of hair in a large population sample,” Forensic Science International, vol. 170, no. 2-3, pp. 121-128, 2007.

  • Related Research Results (1)
  • Metrics
    No metrics available
Share - Bookmark