Making connections: snapshots of chlamydial type III secretion systems in contact with host membranes

Article English OPEN
Dumoux, Maud ; Nans, Andrea ; Saibil, Helen R. ; Hayward, Richard D. (2015)
  • Publisher: Elsevier
  • Related identifiers: doi: 10.1016/j.mib.2014.09.019
  • Subject: bcs | Bacterial Secretion Systems, Cell Membrane, Chlamydia, Endocytosis, Macromolecular Substances, Models, Biological

Chlamydiae are obligate intracellular bacterial pathogens with an unusual biphasic lifecycle, which is underpinned by two bacterial forms of distinct structure and function. Bacterial entry and replication require a type III secretion system (T3SS), a widely conserved nanomachine responsible for the translocation of virulence effectors into host cells. Recent cell biology experiments supported by electron and cryo-electron tomography have provided fresh insights into Chlamydia-host interactions. In this review, we highlight some of the recent advances, particularly the in situ analysis of T3SSs in contact with host membranes during chlamydial entry and intracellular replication, and the role of the host rough endoplasmic reticulum (rER) at the recently described intracellular 'pathogen synapse'.
  • References (54)
    54 references, page 1 of 6

    References and recommended reading LePine P: Recent research on the trachoma virus. Rev Int Trach 1963, 40:296-308.

    Moulder JW: The relation of the psittacosis group (Chlamydiae) to bacteria and viruses. Ann Rev Microbiol 1966, 20:107-130.

    Hu VH, Holland MJ, Burton MJ: Trachoma: protective and pathogenic ocular immune responses to Chlamydia trachomatis. PLoS Negl Trop Dis 2013, 7:e2020.

    Wang Y, Kahane S, Cutcliffe LT, Skilton RJ, Lambden PR, Persson K, Bjartling C: Clarke IN genetic transformation of a 6. Ding H, Gong S, Tian Y, Yang Z, Brunham R, Zhong G: Transformation of sexually transmitted infection-causing serovars of Chlamydia trachomatis using Blasticidin for selection. PLoS One 2013, 8:e80534.

    Scidmore MA: Cultivation and laboratory maintenance of Chlamydia trachomatis. Curr Protoc Microbiol 2005, Ch 11:Unit 11a.

    Cocchiaro JL, Valdivia RH: New insights into Chlamydia intracellular survival mechanisms. Cell Microbiol 2009, 11:1571-1578.

    Huang Z, Chen M, Li K, Dong X, Han J, Zhang Q: Cryo-electron tomography of Chlamydia trachomatis gives a clue to the mechanism of outer membrane changes. J Electron Microsc (Tokyo) 2010, 59:237-241.

    10. Newhall WJ, Jones RB: Disulphide-linked oligomers of the major outer membrane protein of Chlamydiae. J Bacteriol 1983, 154:998-1001.

    11. Hackstadt T, Todd WJ, Caldwell HD: Disulphide-mediated interactions of the chlamydial outer membrane protein: role in the differentiation of Chlamydiae. J Bacteriol 1985, 161:25-31.

    12. Peters J, Wilson DP, Myers G, Timms P, Bavoil PM: Type III secretion a´ la Chlamydia. Trends Microbiol 2007, 15:241-251.

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