Altering peptide fibrillization by polymer conjugation

Article English OPEN
Dehn, Sabrina ; Castelletto, Valeria ; Hamley, Ian W. ; Perrier, Sébastien (2012)

A strategy is presented that exploits the ability of\ud synthetic polymers of different nature to disturb the strong selfassembly\ud capabilities of amyloid based β-sheet forming peptides.\ud Following a convergent approach, the peptides of interest were\ud synthesized via solid-phase peptide synthesis (SPPS) and the\ud polymers via reversible addition−fragmentation chain transfer\ud (RAFT) polymerization, followed by a copper(I) catalyzed azide−\ud alkyne cycloaddition (CuAAC) to generate the desired peptide−\ud polymer conjugates. This study focuses on a modified version of\ud the core sequence of the β-amyloid peptide (Aβ), Aβ(16−20) (KLVFF). The influence of attaching short poly(Nisopropylacrylamide)\ud and poly(hydroxyethylacrylate) to the peptide sequences on the self-assembly properties of the hybrid\ud materials were studied via infrared spectroscopy, TEM, circular dichroism and SAXS. The findings indicate that attaching these\ud polymers disturbs the strong self-assembly properties of the biomolecules to a certain degree and permits to influence the\ud aggregation of the peptides based on their β-sheets forming abilities. This study presents an innovative route toward targeted and\ud controlled assembly of amyloid-like fibers to drive the formation of polymeric nanomaterials.
  • References (22)
    22 references, page 1 of 3

    (1) Klok, H. A. J. Polym. Sci. A 2005, 43, 1-17.

    (2) Drotleff, S.; Lungwitz, U.; Breunig, M.; Dennis, A.; Blunk, T.; Tessmar, J.; Göpferich, A. European Journal of Pharmaceutics and Biopharmaceutics 2004, 58, 385-407.

    (3) Klok, H.-A. Macromolecules 2009, 42, 7990-8000.

    (4) Rösler, A.; Klok, H. A.; Hamley, I. W.; Castelletto, V.; Mykhaylyk, O. O.

    Biomacromolecules 2003, 4, 859-863.

    (5) Hamley, I. W. Angew. Chem. Int. Ed. 2007, 46, 8128-8147.

    (6) Hartgerink, J. D.; Beniash, E.; Stupp, S. I. Science 2001, 294, 1684-1688.

    (7) Reches, M.; Gazit, E. Science 2003, 300, 625-627.

    (8) Yang, Y. L.; Khoe, U.; Wang, X. M.; Horii, A.; Yokoi, H.; Zhang, S. G. Nano Today 2009, 4, 193-210.

    (9) Ulijn, R. V.; Smith, A. M. Chem. Soc. Rev. 2007, 37, 664-675.

  • Metrics
    0
    views in OpenAIRE
    0
    views in local repository
    44
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    Central Archive at the University of Reading - IRUS-UK 0 44
Share - Bookmark