Neonatal development of the corneal stroma in wild-type and lumican-null mice

Article English OPEN
Beecher, Nicola ; Chakravarti, S. ; Joyce, S. ; Meek, Keith Michael Andrew ; Quantock, Andrew James (2006)
  • Publisher: Association for Research in Vision and Ophthalmology
  • Related identifiers: doi: 10.1167/iovs.05-0907
  • Subject: RE
    mesheuropmc: sense organs

PURPOSE: Between days 8 and 14 of neonatal development, the corneal stroma of the mouse undergoes critical changes in tissue thickness, cell density, and light scattering. The authors investigate the stromal matrix structure in wild-type and lumican-deficient corneas in this developmental phase.\ud \ud METHODS: Wild-type (n = 44) and lumican-deficient (n = 42) mouse corneas at neonatal days 8, 10, 12, and 14 were investigated by synchrotron x-ray diffraction to establish the average collagen fibril spacing, average collagen fibril diameter, and level of fibrillar organization in the stromal matrix. RESULTS: Collagen interfibrillar spacing in the normal mouse cornea became more closely packed between days 8 and 14, though not significantly so. In lumican-null mice, interfibrillar spacing was significantly elevated at days 8, 10, and 12, but not day 14, compared with that in wild-type mice. At all stages investigated, collagen fibrils were, on average, marginally thinner than normal in lumican-null mutants, and the spatial distribution of the fibrils was less well organized.\ud \ud CONCLUSIONS: Transient thickening of the corneal stroma of the normal mouse at eye opening is probably not caused by widespread, homogeneous rearrangement of collagen fibrils but more likely by a temporary increase in cell or stromal "lake" volume. Lumican, structurally influential in adult mouse corneas, is also a key molecule in the neonatal development of the stromal matrix.
  • References (28)
    28 references, page 1 of 3

    1. Maurice DM. The structure and transparency of the cornea. J Physiol. 1957;136:263-286.

    2. Anseth A. Glycosaminoglycans in corneal regeneration. Exp Eye Res. 1961;1:122-127.

    3. Hassell JR, Cintron C, Kublin C, Newsome DA. Proteoglycan changes during restoration of transparency in corneal scars. Arch Biochem Biophys. 1983;222:362-369.

    4. Nakazawa K, Hassell JR, Hascall VC, Lohmander LS, Newsome DA, Krachmer J. Defective processing of keratan sulfate in macular corneal dystrophy. J Biol Chem. 1984;259:22:13751-13757.

    5. Yang CJ, SundarRaj N, Thonar J-Ma, Klintworth GK. Immunohistochemical evidence of heterogeneity in macular corneal dystrophy. Am J Ophthalmol. 1988;106:65-71.

    6. Quantock AJ, Meek KM, Ridgway AEA, Bron AJ, Thonar EJ-MA. Macular corneal dystrophy: reduction in both corneal thickness and collagen interfibrillar spacing. Curr Eye Res. 1990;9:4:393- 398.

    7. Anseth A. Glycosaminoglycans in the developing corneal stroma. Exp Eye Res. 1961;1:116 -121.

    8. Cornuet PK, Blochberger TC, Hassell JR. Molecular polymorphism of lumican during corneal development. Invest Ophthalmol Vis Sci. 1994;35:870 - 877.

    9. Connon CJ, Meek KM, Kinoshita S, Quantock AJ. Spatial and temporal alterations in the collagen fibrillar array during the onset of transparency in the avian cornea. Exp Eye Res. 2004;78:909 - 915.

    10. Blochberger TC, Vergnes JP, Hempel J, Hassell JR. cDNA to chick lumican (corneal keratan sulfate proteoglycan) reveals homology to the small interstitial proteoglycan gene family and expression in muscle and intestine. J Biol Chem. 1992;267:1:347-352.

  • Similar Research Results (1)
  • Metrics
    0
    views in OpenAIRE
    0
    views in local repository
    14
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    Online Research @ Cardiff - IRUS-UK 0 14
Share - Bookmark