Controlling the rheology of gellan gum hydrogels in cell culture conditions

Article English OPEN
Moxon, Samuel R. ; Smith, Alan M. (2016)

Successful culturing of tissues within polysaccharide hydrogels is reliant upon specific mechanical properties. Namely, the stiffness and elasticity of the gel have been shown to have a profound effect on cell behaviour in 3D cell cultures and correctly tuning these mechanical properties is critical to the success of culture. The usual way of tuning mechanical properties of a hydrogel to suit tissue engineering applications is to change the concentration of polymer or its cross-linking agents. In this study sonication applied at various amplitudes was used to control mechanical properties of gellan gum solutions and gels. This method enables the stiffness and elasticity of gellan gum hydrogels cross-linked with DMEM to be controlled without changing either polymer concentration or cross-linker concentration. Controlling the mechanical behaviour of gellan hydrogels impacted upon the activity of alkaline phosphatase (ALP) in encapsulated MC3T3 pre-osteoblasts. This shows the potential of applying a simple technique to generate hydrogels where tissue-specific mechanical properties can be produced that subsequently influence cell behaviour.
  • References (29)
    29 references, page 1 of 3

    [1] Drury, J.L. Dennis, R.G. Mooney, D.J. (2004). The tensile properties of alginate hydrogels, Biomaterials, 25, 3187-3199.

    [2] Hishikawa, K. Miura, S. Marumo, T. Yoshioka, H. Mori, Y. Takato, T. Fujita, T. (2004). Gene expression profile of human mesenchymal stem cells during osteogenesis in threedimensional thermoreversible gelation polymer, Biochemical and Biophysical Research Communications, 317 (4), 1103-1107.

    [3] Fan, J. Gong, Y. Ren, L. Varshney, R.R. Cai, D. Wang, D.A. (2010). In vitro engineered cartilage using synovium-derived mesenchymal stem cells with injectable gellan hydrogels, Acta Biomaterialia, 6, 1178-1185.

    [4] Park, H. Temenoff, J.S. Tabata, Y. Caplan, A.I. Mikos, A.G. (2007). Injectable biodegradable hydrogel composites for rabbit marrow mesenchymal stem cell and growth factor delivery for cartilage tissue engineering, Biomaterials, 28 (21), 3217-3227.

    [5] Kim, J. Kim, I.S. Cho, T.H. Lee, K.B. Hwang, S.J. Tae, G. Noh, I. Lee, S.H. Park, Y. Sun, K. (2007). Bone regeneration using hyaluronic acid-based hydrogel with bone morphogenic protein-2 and human mesenchymal stem cells, Biomaterials, 28 (10), 1830- 1837.

    [6] Drury, J.L. Mooney, D.J. (2003). Hydrogels for tissue engineering: scaffold design variables and applications, Biomaterials, 24 (24), 4337-4351.

    [7] Du, H. Hamilton, P. Reilly, M. Ravi, N. (2012). Injectable in situ physically and chemically crosslinkable gellan hydrogel, Macromolecular Bioscience, 12, 952-961.

    [8] Osmalek, T. Froelich, A. Tasarek, S. (2014). Application of gellan gum in pharmacy and medicine, International Journal of Pharmaceutics, 466 (1-2), 328-340.

    [9] Smith, AM. Shelton, R.M. Perrie, Y. Harris, J.J. (2007). An initial evaluation of gellan gum as a material for tissue engineering applications, Journal of Biomaterial Applications, 22 (3), 241-254.

    [10] Jahromi, S.H. Grover, L.M. Paxton, J.Z. Smith, A.M, (2011). Degradation of polysaccharide hydrogels seeded with bone marrow stromal cells, Journal of the Mechanical Behavior of Biomedical Materials, 4 (7), 1157-1166.

  • Metrics
    0
    views in OpenAIRE
    0
    views in local repository
    17
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    University of Huddersfield Repository - IRUS-UK 0 17
Share - Bookmark