Application of DIGE and mass spectrometry in the study of type 2 Diabetes Mellitus (T2DM) mouse models

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Smith, Celia ; Mills, Davinia ; Cramer, Rainer (2012)

Knowledge of the differences between the amounts and types of protein that are expressed in diseased compared to healthy subjects may give an understanding of the biological pathways that cause disease. This is the reasoning behind the presented protocol, which uses difference gel electrophoresis to discover up‐ or down‐regulated proteins between mice of different genotypes, or\ud of those fed on different diets, that may thus be prone to develop diabetes‐like phenotypes. Subsequent analysis of these proteins by tandem mass spectrometry typically facilitates their identification with a high degree of confidence.
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    1. Rehydration tray and lid (GE Healthcare)  2. 2 pairs of tweezers  3. Thin but blunt long‐bladed spatula  4. Microwave oven  5. Ettan DALTtwelve separation unit (GE Healthcare)  6. Rehydration tray and lid (GE Healthcare) 

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