Piliation of Invasive Streptococcus pneumoniae Isolates in the Era before Pneumococcal Conjugate Vaccine Introduction in Malawi

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Kulohoma, Benard W. ; Gray, Katherine ; Kamng'ona, Arox ; Cornick, Jennifer ; Bentley, Stephen D. ; Heyderman, Robert S. ; Everett, Dean B. (2013)
  • Publisher: American Society for Microbiology
  • Journal: Clinical and Vaccine Immunology : CVI, volume 20, issue 11, pages 1,729-1,735 (issn: 1556-6811, eissn: 1556-679X)
  • Related identifiers: pmc: PMC3837777, doi: 10.1128/CVI.00403-13
  • Subject: wc_210 | qw_551 | Vaccines | qu_475 | qw_805 | wc_217
    mesheuropmc: biochemical phenomena, metabolism, and nutrition | bacteria

The pneumococcal pilus has been shown to be an important determinant of adhesion and virulence in mouse models of colonization, pneumonia, and bacteremia. A pilus is capable of inducing protective immunity, supporting its inclusion in next-generation pneumococcal protein vaccine formulations. Whether this vaccine target is common among pneumococci in sub-Saharan Africa is uncertain. To define the prevalence and genetic diversity of type I and II pili among invasive pneumococci in Malawi prior to the introduction of the 13-valent pneumococcal conjugate vaccine (PCV13) into routine childhood immunization, we examined 188 Streptococcus pneumoniae isolates collected between 2002 and 2008 (17% serotype 1). In this region of high disease burden, we found a low frequency of invasive piliated pneumococci (14%) and pilus gene sequence diversity similar to that seen previously in multiple global pneumococcal lineages. All common serotypes with pilus were covered by PCV13 and so we predict that pilus prevalence will be reduced in the Malawian pneumococcal population after PCV13 introduction.
  • References (37)
    37 references, page 1 of 4

    1. O'Brien KL, Wolfson LJ, Watt JP, Henkle E, Deloria-Knoll M, McCall N, Lee E, Mulholland K, Levine OS, Cherian T. 2009. Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: global estimates. Lancet 374:893-902.

    2. Bogaert D, De Groot R, Hermans PW. 2004. Streptococcus pneumoniae colonisation: the key to pneumococcal disease. Lancet Infect. Dis. 4:144 - 154.

    3. Jedrzejas MJ. 2001. Pneumococcal virulence factors: structure and function. Microbiol. Mol. Biol. Rev. 65:187-207.

    4. Ton-That H, Schneewind O. 2004. Assembly of pili in Gram-positive bacteria. Trends Microbiol. 12:228 -234.

    5. Lauer P, Rinaudo CD, Soriani M, Margarit I, Maione D, Rosini R, Taddei AR, Mora M, Rappuoli R, Grandi G, Telford JL. 2005. Genome analysis reveals pili in group B Streptococcus. Science 309:105.

    6. Mora M, Bensi G, Capo S, Falugi F, Zingaretti C, Manetti AG, Maggi T, Taddei AR, Grandi G, Telford JL. 2005. Group A Streptococcus produce pilus-like structures containing protective antigens and Lancefield T antigens. Proc. Natl. Acad. Sci. U. S. A. 102:15641-15646.

    7. Alteri CJ, Xicohtencatl-Cortes J, Hess S, Caballero-Olin G, Giron JA, Friedman RL. 2007. Mycobacterium tuberculosis produces pili during human infection. Proc. Natl. Acad. Sci. U. S. A. 104:5145-5150.

    8. Barocchi MA, Ries J, Zogaj X, Hemsley C, Albiger B, Kanth A, Dahlberg S, Fernebro J, Moschioni M, Masignani V, Hultenby K, Taddei AR, Beiter K, Wartha F, von Euler A, Covacci A, Holden DW, Normark S, Rappuoli R, Henriques-Normark B. 2006. A pneumococcal pilus influences virulence and host inflammatory responses. Proc. Natl. Acad. Sci. U. S. A. 103:2857-2862.

    9. Bagnoli F, Moschioni M, Donati C, Dimitrovska V, Ferlenghi I, Facciotti C, Muzzi A, Giusti F, Emolo C, Sinisi A, Hilleringmann M, Pansegrau W, Censini S, Rappuoli R, Covacci A, Masignani V, Barocchi MA. 2008. A second pilus type in Streptococcus pneumoniae is prevalent in emerging serotypes and mediates adhesion to host cells. J. Bacteriol. 190: 5480 -5492.

    10. Telford JL, Barocchi MA, Margarit I, Rappuoli R, Grandi G. 2006. Pili in Gram-positive pathogens. Nat. Rev. Microbiol. 4:509 -519.

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