publication . Article . 2016

A novel regulatory role for tissue transglutaminase in epithelial-mesenchymal transition in cystic fibrosis

Nyabam, Samuel; Wang, Zhuo; Thibault, Thomas; Ayinde, Oluseyi; Basar, Rameeza; Marshall, Lindsay; Griffin, Martin;
Open Access English
  • Published: 01 Sep 2016
  • Country: United Kingdom
Abstract
Cystic fibrosis (CF) is a genetic disorder caused by mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) for which there is no overall effective treatment. Recent work indicates tissue transglutaminase (TG2) plays a pivotal intracellular role in proteostasis in CF epithelia and that the pan TG inhibitor cysteamine improves CFTR stability. Here we show TG2 has another role in CF pathology linked with TGFβ1 activation and signalling, induction of epithelial-mesenchymal transition (EMT), CFTR stability and induction of matrix deposition. We show that increased TG2 expression in normal and CF bronchial epithelial cells increases TGFβ1 levels, ...
Related Organizations
Funded by
EC| TRANSCOM
Project
TRANSCOM
The Commercialisation of Transglutaminase.
  • Funder: European Commission (EC)
  • Project Code: 251506
  • Funding stream: FP7 | SP3 | PEOPLE
43 references, page 1 of 3

[1] L. Maiuri, A. Luciani, I. Giardino, V. Raia, V.R. Villella, MR.D'Apolito, M. PettoelloMantovani, S. Guido, C. Ciacci, M. Cimmino, O.N. Cexus, M. Londei, S. Quaratino, Tissue transglutaminase activation modulates inflammaCtion in cystic fibrosis via PPARgamma down-regulation, Journal of immunology, 1S80 (2008) 7697-7705. [OpenAIRE]

[2] N. Regamey, P.K. Jeffery, E.W. Alton, A. Bush, J.C. Davies, Airway remodelling and its relationship to inflammation in cystic fibrosis, ThoUrax, 66 (2011) 624-629. [OpenAIRE]

[3] J. Shute, L. Marshall, K. Bodey, A. Bush, Growth factors in cystic fibrosis - when more is not enough, Paediatric respiratory reviews, N4(2003) 120-127.

[4] S.M. Snodgrass, K.M. Cihil, P.K. CornueAt,M.M. Myerburg, A. Swiatecka-Urban, Tgf-beta1 inhibits Cftr biogenesis and prevents functional rescue of DeltaF508-Cftr in primary differentiated human bronchial epitMhelial cells, PloS one, 8 (2013) e63167. [OpenAIRE]

[5] M. Wilschanski, Novel therapeutic approaches for cystic fibrosis, Discovery medicine, 15 (2013) 127-133. D

[6] F. Van Goor, S. Hadida, P.D. Grootenhuis, B. Burton, D. Cao, T. Neuberger, A. Turnbull, A. Singh, J. Joubran, A. HEazlewood, J. Zhou, J. McCartney, V. Arumugam, C. Decker, J. Yang, C. Young, E.TR. Olson, J.J. Wine, R.A. Frizzell, M. Ashlock, P. Negulescu, Rescue of CF airway epithelial cell function in vitro by a CFTR potentiator, P VX-770, Proceedings of the National Academy of Sciences of the United States of America, 106 (2009) 18825E-18830. [OpenAIRE]

[7] D. De Stefano, V.R. Villella, S. Esposito, A. Tosco, A. Sepe, F. De Gregorio, L. Salvadori, R. Grassia, C.CA.Leone, G. De Rosa, M.C. Maiuri, M. Pettoello-Mantovani, S. Guido, A. Bossi, A. CZolin, A. Venerando, L.A. Pinna, A. Mehta, G. Bona, G. Kroemer, L. Maiuri, V. Raia, Restoration of CFTR function in patients with cystic fibrosis carrying the F508del-CFTR Amutation, Autophagy, 10 (2014) 2053-2074. [OpenAIRE]

[8] Z. Wang, R.J. Collighan, K. Pytel, D.L. Rathbone, X. Li, M. Griffin, Characterization of heparin-binding site of tissue transglutaminase: its importance in cell surface targeting, matrix deposition, and cell signaling, J Biol Chem, 287 (2012) 13063-13083. [OpenAIRE]

[9] E. Verderio, S.R. Gross, M. Griffin, Cell-surface tissue transglutaminase regulates matrix storage of latent TGF-beta binding protein-1 (LTBP-1) and fibronectin accumulation, Mol. Biol. Cell, 10 (1999) 370.

[10] C. Niger, K.E. Beazley, M. Nurminskaya, Induction of chondrogenic differentiation in mesenchymal stem cells by TGF-beta cross-linked to collagen-PLLA [poly(L-lactic acid)] scaffold by transglutaminase 2, Biotechnology letters, 35 (2013) 2193-2199. [OpenAIRE]

[11] W.T. Harris, M.S. Muhlebach, R.A. Oster, M.R. Knowles, T.L. Noah, Transforming growth factor-beta(1) in bronchoalveolar lavage fluid from children with cystic fibrosis, Pediatric pulmonology, 44 (2009) 1057-1064.

[12] S. Kumar, K. Mehta, Tissue transglutaminase, inflammation, and cancer: how intimate is the relationship?, Amino Acids, 44 (2013) 81-88.

[13] S.P. Srivastava, D. Koya, K. Kanasaki, MicroRNAs in kidney fibrosis and diabetic nephropathy: roles on EMT and EndMT, BioMed research international, 2013 (2013) 125469.

[14] Z. Wang, M. Perez, S. Caja, G. Melino, T.S. Johnson, K. Lindfors, M. Griffin, A T novel extracellular role for tissue transglutaminase in matrix-bound VEGF-mediated angiogenesis, Cell death & disease, 4 (2013) e808. P

[15] H.G. Baumgarten, Biogenic monoamines in the cyclostomeIand lower vertebrate brain, Progress in histochemistry and cytochemistry, 4 (1972) 1R-90.

43 references, page 1 of 3
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue