Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates

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Cellier, Marie ; James, Arthur ; Lowe, Jonathan ; Orenga, Sylvain ; Perry, John ; Rasul, Ari ; Stanforth, Stephen (2016)

A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying l-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.
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