Interaction specificity of Arabidopsis 14-3-3 proteins with phototropin receptor kinases

Article English OPEN
Sullivan, Stuart ; Thompson, Catriona E. ; Kaiserli, Eirini ; Christie, John M. (2009)

Phototropin receptor kinases play an important roles in optimising plant growth in response to blue light. Much is known regarding their photochemical reactivity, yet little progress has been made to identify downstream signalling components. Here, we isolated several interacting proteins for Arabidopsis phototropin 1 (phot1) by yeast two-hybrid screening. These include members of the NPH3/RPT2 (NRL) protein family, proteins associated with vesicle trafficking, and the 14-3-3 lambda (?) isoform from Arabidopsis . 14-3-3? and phot1 were found to colocalise and interact in vivo. Moreover, 14-3-3 binding to phot1 was limited to non-epsilon 14-3-3 isoforms and was dependent on key sites of receptor autophosphorylation. No 14-3-3 binding was detected for Arabidopsis phot2, suggesting that 14-3-3 proteins represent specific mode of phot1 signalling.
  • References (40)
    40 references, page 1 of 4

    [1] Christie, J.M. (2007) Phototropin blue-light receptors. Annu. Rev. Plant Biol. 58, 21-45.

    [2] Kinoshita, T., Doi, M., Suetsugu, N., Kagawa, T., Wada, M. and Shimazaki, K. (2001) Phot1 and phot2 mediate blue light regulation of stomatal opening. Nature 414, 656-660.

    [3] Kagawa, T., Sakai, T., Suetsugu, N., Oikawa, K., Ishiguro, S., Kato, T., Tabata, S., Okada, K. and Wada, M. (2001) Arabidopsis NPL1: a phototropin homolog controlling the chloroplast high-light avoidance response. Science 291, 2138- 2141.

    [4] Takemiya, A., Inoue, S., Doi, M., Kinoshita, T. and Shimazaki, K. (2005) Phototropins promote plant growth in response to blue light in low light environments. Plant Cell 17, 1120-1127.

    [5] Inoue, S., Kinoshita, T., Takemiya, A., Doi, M. and Shimazaki, K. (2008) Leaf positioning of Arabidopsis in response to blue light. Mol. Plant 1, 1-12.

    [6] Huala, E., Oeller, P.W., Liscum, E., Han, I.-S., Larsen, E. and Briggs, W.R. (1997) Arabidopsis NPH1: a protein kinase with a putative redox-sensing domain. Science 278, 2120-2123.

    [7] Christie, J.M., Salomon, M., Nozue, K., Wada, M. and Briggs, W.R. (1999) LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1): Binding sites for the chromophore flavin mononucleotide. Proc. Natl. Acad. Sci. USA 96, 8779-8783.

    [8] Christie, J.M., Swartz, T.E., Bogomolni, R.A. and Briggs, W.R. (2002) Phototropin LOV domains exhibit distinct roles in regulating photoreceptor function. Plant J. 32, 205-219.

    [9] Cho, H.Y., Tseng, T.S., Kaiserli, E., Sullivan, S., Christie, J.M. and Briggs, W.R. (2007) Physiological roles of the light, oxygen, or voltage domains of phototropin 1 and phototropin 2 in Arabidopsis. Plant Physiol. 143, 517-529.

    [10] Nakasako, M., Zikihara, K., Matsuoka, D., Katsura, H. and Tokutomi, S. (2008) Structural basis of the LOV1 dimerization of Arabidopsis phototropins 1 and 2. J. Mol. Biol. 381, 718-733.

  • Metrics
    No metrics available
Share - Bookmark