Optimizing immunoslot blot assays and application to low DNA adduct levels using an amplification approach

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Moore, SA; Xeniou, O; Zeng, ZT; Humphreys, E; Burr, S; Gottschalg, E; Bingham, SA; Shuker, DEG;

Immunoslot blot assays have been used for the analysis of many DNA adducts, but problems are frequently encountered in achieving reproducible results. Each step of the assay was examined systematically, and it was found that the major problems are in the DNA fragmentati... View more
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