publication . Thesis . 1974

A cytochemical and biochemical study of acid hydrolases in developing plant cells, with particular reference to non-specific esterases

James, David John;
Open Access English
  • Published: 01 Feb 1974
  • Country: United Kingdom
Abstract
Investigations have been carried out into the occurrence and functions of acid hydrolases during plant cell division, differentiation and senescence using histochemical and biochemical procedures. Statistical analyses of histochemical data have shown that particles staining for naphthol AS BI phosphatase in dividing unfixed root cells of Vicia f aba do not act as a 'trigger 1 in the way suggested for animal cells, since there were significantly greater numbers of particles in mitotic cells as opposed to interphase cells in all 3 root tissues, apical initials, procortex and central cylinder. Increasing the length of incubation time markedly increased the number o...
Subjects
Medical Subject Headings: food and beverages
free text keywords: SB
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26 references, page 1 of 2

MATILE, P., (1966): Enzyme der Vakuolen aus Worzelzellen von Maiskeimlingen. Ein Beitrag zur funktionellen Bedeutung der Vakuole bei der intrazellularen Verdauung. Z. Naturforsch. 21b, 871-878.

McLEAN, J., (1970): A histochemical study of acid hydrolases in dividing and differentiating plant tissue. Ph. D. Thesis. Univ.

PEARSE, A.G.E., (i960): Histochemistry, theoretical and applied.

Vol. 1. London A. Churchill Ltd.

PEARSE, A. G.E., (1973): Histochemistry, thoretical and applied.

Vol. 2. London. A. Churchill Ltd.

SACHER, S.A., (1967): Studies of permeability, RNA and protein turnover during ageing of fruit and leaf tissues. In: Aspects of the Biology of Ageing, ed. H.W. Woolhouse. S.E.B. Symposium.

Vol. XXI. pp. 269-303. Academic Press. New York.

SHAW, M. & MANACHA, M.S., (1965): Fine structure in detached senescing wheat leaves. Can. J. Bot., £2, 747-755.

WALEK-CZERNECKA, A., (1962): Note sur la detection d'une esterase non-specifique dans les spherosomes. Acta. Soc. Bot. Pol., 32, 405-408.

WOODING, F.B.P. & NCRTHCOTE, D.H., (1964): The development of the secondary wall of the xylem in Acer pseudoplatanus. J. Cell Biol. , 22, 237-337. [OpenAIRE]

Fig. 2A D. XL V. Spectra of L-ascorbate, fast red violet LB salt and mixtures thereof.

Cuvettes of 1 cm. path length contained: A) 0.067 mM. L-ascorbate; B) 30 [Ag/ml of fast red violet LB salt; C) 30 [jig/ml of fast red violet LB salt and 0.033 mM L-ascorbate; D) 30 [ig/ml of fast red violet LB salt and 0.008 mM L-ascorbate, all in Mcllvaine's buffer pH 5.4. The reference cuvette contained the same buffer

Appleton, T. C.: The possibilities of locating soluble labeled compounds by electron microscope autoradiography. In: Autoradiography of diffusible substances, p. 304-320 (L. J. Roth and W. E. Stumpf, eds.). New York and London: Academic Press 1969.

Bernhard, W., Leduc, E. H.: Ultra-thin frozen sections. I. Methods and ultra-structural preservation. J. Cell Biol. 34, 757-771 (1967). [OpenAIRE]

26 references, page 1 of 2
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