Prussian Blue acts as a mediator in a reagentless cytokinin biosensor

Article English OPEN
Kowalska, Marta ; Tian, Faming ; Šmehilová, Mária ; Galuszka, Petr ; Frébort, Ivo ; Napier, R. (Richard) ; Dale, Nicholas (2011)
  • Publisher: Elsevier Science BV
  • Related identifiers: doi: 10.1016/j.aca.2011.06.018
  • Subject: QK
    mesheuropmc: food and beverages | fungi | macromolecular substances | heterocyclic compounds

An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N(6)-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol-gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface.
  • References (44)
    44 references, page 1 of 5

    [1] O. Hedden, Ann. Rev. Plant Physiol. Plant Mol. Biol. 44 (1993) 107-130.

    [2] G.C. Davis, M.B. Hein, B.C. Neely, C.R. Sharp, M.G. Carnes, Anal. Chem. 63 (1985) 639A648A.

    [3] J. Olsson, K. Claeson, B. Karlberg, A.C. Nordström, J. Chromatogr. A 824 (1998) 231-239.

    [4] B. Sotta, G. Pillate, F. Pelese, I. Sabbagh, M. Bonnet, R. Maldiney, Plant Physiol. 84 (1987) 571- 573.

    [5] H. Wabiko, M. Minemura, Plant Physiol. 112 (1996) 939-951.

    [6] L. River, A. Crozier, Principles and Practice of Hormone Analysis, Academic Press, London, 1987

    [7] E.W. Weiler, J. Eberle, R. Mertens, R. Atzon, M. Feyerabend, P.S. Jordan, A. Arnscheidt, U. Wieczorek, Immunology in Plant Science, Cambridge Press, Cambridge, 1986.

    [8] E.M.S. MacDonail, R.O. Morris, Methods Ezymol. 110 (1985) 347-358.

    [9] L. Clark, C. Lyons, Ann. N. Y. Acad. Sci. 148 (1962) 133-153.

    [10] M. Pohanka, P. Skládal, J. Applied Biomed. 6 (2008) 57-64.

  • Metrics
    0
    views in OpenAIRE
    0
    views in local repository
    75
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    Warwick Research Archives Portal Repository - IRUS-UK 0 75
Share - Bookmark