Structured illumination microscopy for in-vivo human retinal imaging: a theoretical assessment

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Chetty, S. ; Gruppetta, S. (2012)

Structured illumination microscopy applied to in-vivo retinal imaging has the potential to provide a low-cost and powerful diagnostic tool for retinal disease. In this paper the key parameters that affect performance in structured illumination ophthalmoscopy are studied theoretically. These include the number of images that need to be acquired in order to generate a sectioned image, which is affected by the non-stationary nature of the retina during acquisition, the choice of spatial frequency of the illuminating sinusoid, the effect of typical ocular aberrations on axial resolution and the nature of the sinusoidal pattern produced by the illumination system. The results indicate that structured illumination ophthalmoscopy can be a robust technique for achieving axial sectioning in retinal imaging without the need for complex optical systems.
  • References (15)
    15 references, page 1 of 2

    1. M. A. A. Neil, R. Juskaitis, and T. Wilson, “Method of obtaining optical sectioning by using structured light in a conventional microscope,” Optics Letters 22(24), 1905-1907 (1997).

    2. D. Karadaglic´, “Image formation in conventional brightfield reflection microscopes with optical sectioning via structured illumination,” Micron 39, 302-310 (2008).

    3. D. Karadaglic´ and T. Wilson, “Image formation in structured illumination wide-field fluorescence microscopy,” Micron 39, 808-818 (2008).

    4. M. G. L. Gustafsson, “Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution,” Proceedings of the National Academy of Science 102(37), 13,081-13,086 (2005).

    5. P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. L. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nature Methods 6, 339-342 (2009).

    6. S. Gruppetta and S. Chetty, “Theoretical study of multispectral structured illumination for depth resolved imaging of non-stationary objects: focus on retinal imaging,” Biomedical Optics Express 2(2), 255-263 (2011).

    7. S. Gruppetta, “Optical Imaging System,” WO Patent WO/2012/059564, PCT/EP2011/069375 (2011).

    8. D. Karadaglic´, “Wide-field Optical Sectioning Microscopy using Structured Illumination,” Ph.D. thesis, University of Oxford (2004).

    9. N. Hagan, L. Gao, and T. S. Tkaczyk, “Quantitative sectioning and noise analysis for structured illumination microscopy,” Optics Express 20(1), 403-413 (2012).

    10. T. Wilson and C. Sheppard, eds., Theory and Practice of Scanning Optical Microscopy (Academic Press, 1984).

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