Modeling enzyme processivity reveals that RNA-Seq libraries are biased in characteristic and correctable ways\ud \ud

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Archer, Nathan; Walsh, Mark; Shahrezaie, Vahid; Hebenstreit, Daniel;

Experimental procedures for preparing RNA-seq and single-cell (sc) RNA-seq libraries are based on assumptions regarding their underlying enzymatic reactions. Here, we show that the fairness of these assumptions varies within libraries: coverage by sequencing reads along... View more
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