publication . Article . Other literature type . 2012

The cytotoxicity of polycationic iron oxide nanoparticles: Common endpoint assays and alternative approaches for improved understanding of cellular response mechanism

Clare Hoskins; Alfred Cuschieri; Lijun Wang;
Open Access English
  • Published: 01 Apr 2012
  • Publisher: BioMed Central
  • Country: United Kingdom
Abstract
<p>Abstract</p> <p>Background</p> <p>Iron oxide magnetic nanoparticles (MNP's) have an increasing number of biomedical applications. As such in vitro characterisation is essential to ensure the bio-safety of these particles. Little is known on the cellular interaction or effect on membrane integrity upon exposure to these MNPs. Here we synthesised Fe<sub>3</sub>O<sub>4 </sub>and surface coated with poly(ethylenimine) (PEI) and poly(ethylene glycol) (PEG) to achieve particles of varying surface positive charges and used them as model MNP's to evaluate the relative utility and limitations of cellular assays commonly applied for nanotoxicity assessment. An alternat...
Subjects
Medical Subject Headings: technology, industry, and agriculturemacromolecular substances
free text keywords: Q1, R1, Molecular Medicine, Applied Microbiology and Biotechnology, Medicine (miscellaneous), Bioengineering, Pharmaceutical Science, Biomedical Engineering, Research, Magnetic nanoparticle, Cellular interaction, Cell membrane, Cytotoxicity, Cell viability assay, Atomic force microscopy, Zeta potential, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5
Funded by
RCUK| Biomagnetic retraction for endoscopic surgery
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: EP/H010033/1
  • Funding stream: EPSRC
,
RCUK| Magnetic nanoparticles for nerve regeneration
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: EP/H007040/1
  • Funding stream: EPSRC
41 references, page 1 of 3

1. Acar HYc, Garaas RS, Syud F, Bonitatebus P, Kulkarni AM: Superparamagnetic nanoparticles stabilized by polymerized PEGylated coatings. J Magn Magn Mater 2005, 293:1-7. [OpenAIRE]

2. Shuayev VI, Pisanic TR, Jun S: Magnetic nanoparticles for theragnostics. Adv Drug Deliver Rev 2009, 61:467-477. [OpenAIRE]

3. Neuberger T, Schöf B, Hofmann H, Hofmann M, von Rechenberg B: Superparamagnetic nanoparticles for biomedical applications: Possibilities and limitations of a new drug delivery system. J Magn Magn Mater 2005, 293:483-496.

4. Kralj S, Makovec D, Čampelj S, Drofenik M: Producing ultra-thin silica coatings on iron-oxide nanoparticles to improve their surface reactivity. J Magn Magn Mater 2010, 322:1847-1853. [OpenAIRE]

5. Tural B, Özkan N, Volkan M: Preparation and characterisation of polymer coated superparamagnetic magnetite nanoparticle agglomerates. J Phy Chem Solids 2009, 70:860-866. [OpenAIRE]

6. Goon IY, Lim LLMH M, Munroe P, Gooding JJ, Amal R: Fabrication and dispersion of gold-shell0protected magnetite nanoparticles: Systematic control using polyethylenimine. Chem Mater 2009, 21:673-681.

7. Zablotskaya A, Segal I, Maiorov M, Zablotsky D, Mishnev A, Lukevics E, Shestakova I, Domracheva I: Synthesis and characterization of nanoparticles with an iron oxide magnetic core and a biologically active trialkylsiylated aliphatic alkanolamine shell. J Magn Magn Mater 2007, 311:135-139.

8. Pisanic TR, Blackwell JD, Shubayev VI, Fiñones RR, Jin S: Nanotoxicity of iron oxide nanoparticle internalization in growing neurons. Biomaterials 2007, 28:2572-2581. [OpenAIRE]

9. Ying E, Hwang H-M: In vitr evaluation of the cytotoxicity of iron oxide nanoparticles with different coatings and different sizes in A3 human T lymphocytes. Sci Total Environ 2010, 408:4475-4481.

10. Cole AJ, Yang VC, David AE: Cancer theranostics: the rise of targeted magnetic nanoparticles. Trends Biotechnol 2011, 29:323-332.

11. Soenen SJ, Himmerlreich U, Nuytten N, Cuyper MD: Cytotoxic effects of iron oxide nanoparticles and implications for safety in cell labelling. Biomaterials 2011, 32:195-205.

12. Levy M, Luciani N, Alloyeau D, Elgrabi D, Deveaux V, Pechoux C, Chat S, Wang G, Vats N, Gendron F, Factor C, Lotersztajn S, Luciani A, Wilhelm C, Gazeau F: Long term in vivo biotransformation of iron oxide nanoparticles. Biomaterials 2011, 32:3988-3999. [OpenAIRE]

13. Becker C, Hodenius M, Blendinger G, Sechi A, Hieronymus T, MüllerSchulte D, Schmitz-Rode T, Zenke M: Uptake of magnetic nanoparticles into cells for cell tracking. J Magn Magn Mater 2007, 311:234-237.

14. Sorensen M, Autrup H, Moller P, Hertel O, Jensen SS, Vinzents P, Knudsen LE, Loft S: Linking exposure to environmental pollutants with biological effects. Mutat Res 2003, 544:255-271. [OpenAIRE]

15. Zhu M-T, Feng W-Y, Wang B, Wang T-C, Gu Y-Q, Wang M, Wang Y, Ouyang H, Zhao Y-L, Chai Z-F: Comparative study of pulmonary responses to nano- and submicron sized ferric oxide in rats. Toxicology 2008, 247:102-111.

41 references, page 1 of 3
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue