publication . Article . 2016

Liquid MALDI MS Analysis of Complex Peptide and Proteome Samples

Kanjana Wiangnon; Rainer Cramer;
Open Access English
  • Published: 29 Jul 2016
  • Publisher: American Chemical Society
  • Country: United Kingdom
Abstract
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) is well-known to be a powerful technique for the analysis of biological samples. By using glycerol-based liquid support matrices (LSMs) instead of conventional MALDI matrices the power of this technique can be extended further. In this study, we exploited LSMs for the identification of complex samples, i.e. the Lactobacillus proteome and a bovine serum albumin (BSA) digest. Liquid and solid MALDI samples were manually and robotically prepared by coupling a nanoflow high performance liquid chromatography (nanoHPLC) system to an automated MALDI sample spotting device. M...
Subjects
free text keywords: Biochemistry, General Chemistry, Proteome, Protein identification, Peptide mass, Bovine serum albumin, biology.protein, biology, Peptide mapping, Tandem mass spectrometry, Analytical chemistry, Mass spectrometry, Peptide, chemistry.chemical_classification, chemistry, Chromatography
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