publication . Article . Other literature type . 2016

Micromechanical Analysis of the Hyaluronan-Rich Matrix Surrounding the Oocyte Reveals a Uniquely Soft and Elastic Composition

Chen, X; Bonfiglio, R; Banerji, S; Jackson, DG; Salustri, A; Richter, RP;
Open Access
  • Published: 21 Jun 2016 Journal: Biophysical Journal, volume 110, issue 12, pages 2,779-2,789 (issn: 0006-3495, Copyright policy)
  • Publisher: Elsevier BV
  • Country: United Kingdom
Abstract
AbstractThe cumulus cell-oocyte complex (COC) matrix is an extended coat that forms around the oocyte a few hours before ovulation and plays vital roles in oocyte biology. Here, we analyzed the micromechanical response of mouse COC matrix by colloidal-probe atomic force microscopy. We found that the COC matrix is elastic insofar as it does not flow and its original shape is restored after force release. At the same time, the COC matrix is extremely soft. Specifically, the most compliant parts of in vivo and in vitro expanded COC matrices yielded Young’s modulus values of 0.5 ± 0.1 Pa and 1.6 ± 0.3 Pa, respectively, suggesting both high porosity and a large mesh ...
Subjects
free text keywords: Biophysics, Cell Biophysics, Crystallography, Penetration (firestop), Modulus, Indentation, Elastic modulus, Matrix (mathematics), Ultrastructure, Elasticity (economics), Biology, Nanotechnology, Oocyte, medicine.anatomical_structure, medicine
Funded by
EC| JELLY
Project
JELLY
Biomolecular Hydrogels – from Supramolecular Organization and Dynamics to Biological Function
  • Funder: European Commission (EC)
  • Project Code: 306435
  • Funding stream: FP7 | SP2 | ERC
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