A series of novel 8-aminophenoxazin-3-one and 7-aminophenoxazin-3-one chromogens and their corresponding β-alanine derivatives were synthesized and evaluated for their ability to detect β-alanyl aminopeptidase activity in bacteria known to hydrolyse β-alanine derivatize... View more
4.2.5. Individual Bacterial Species Streaked Plates. For each strain tested, a bacterial suspension was prepared in saline 0.85% at a standard inoculum of 0.5 McFarland (1.5 × 108 colony forming units per mL), using a densitometer. Using a sterile loop, 10 μL of the bacterial suspension was inoculated onto a test plate containing a particular substrate. The plates were then incubated aerobically at 37 °C and visual inspections made at 24 h and 48 h of incubation.
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