The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine

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Honeychurch, K. C. ; Hart, J. P. (2012)

Screen-printed carbon electrodes (SPCEs) incorporating the electrocatalyst cobalt phthalocyanine (CoPC) have been successfully used for the manufacture of microband electrodes for the determination of reduced glutathione (GSH). Initially investigations were performed to explore the cyclic voltammetric behaviour of these electrodes with potassium ferrocyanide. It was shown that steady state behaviour occurred which is indicative of radial diffusion at a microelectrode. Current densities of 2173 μAcm-2 were obtained compared to 464.4 μAcm-2 for conventional sized (3 mm x 3 mm) screen-printed electrodes were obtained. At scan rates between 5 and 20 mV/s no significant increase in current response was observed. The application of these microband CoPC–SPCEs, to the measurement of GSH by both chronoamperometry and cyclic voltammetry was investigated. The chronoamperometric response was found to be linear between 50 µM and 8.0 mM with a detection limit of 7.3 µM using an applied potential of +0.25 V (vs. Ag/AgCl).
  • References (34)
    34 references, page 1 of 4

    Pemberton, 2007, Recent developments in screen-printed carbon electrode sensors/biosensors for electrochemical analysis. Chapter 23 in Comprehensive Analytical Chemistry, vol. 49, Eds. S. Alegret, A. Merkoci, R.M. Elsevier B.V., Amsterdam, pp. 497-557.

    J.P. Hart, A.K. Abass, K.C. Honeychurch, R.M. Pemberton, S.L. Ryan, R. Wedge, 2003, Sensors/biosensors, based on screen-printing technology for biomedical applications, Indian Journal of Chemistry Section A-Inorganic Bio-Inorganic Physical Theoretical & Analytical Chemistry, 42, 709-718.

    Pemberton, 2004, Some recent designs and developments of screen-printed carbon electrochemical sensors/biosensors for biomedical, environmental and industrial analyses, Anal. Lett.

    K.C. Honeychurch and J.P. Hart, 2003, Screen-printed electrochemical sensors for monitoring metal pollutants, TrAC, 7/8, 456-469.

    J.-L. Chang and J.-M. Zen, 2006, Fabrication of disposable ultramicroelectrodes: Characterization and applications, Electrochem. Commun. 8, 571-576.

    D.H. Craston, C.P. Jones, D.E. Williams, N. Elmurr, 1991, Microband Electrodes Fabricated By Screen Printing Processes - Applications in Electroanalysis, Talanta, 38, 17-26.

    D.E. Williams, K. Ellis, A. Colville, S.J. Dennison, G. Laguillo, J. Larsen, 1997, Hydrodynamic modulation using vibrating electrodes: Application to electroanalysis, J. Electroanal. Chem. 432, 159-169.

    M. Rochelet-Dequaire, B. Limoges P. Brossier, 2006, Subfemtomolar electrochemical detection of target DNA by catalytic enlargement of the hybridized gold nanoparticle labels, Analyst, 131, 923-929.

    F.J. Rawson, W.M. Purcell, J. Xu, D.C. Cowell, P.R. Fielden, N. Biddle, J.P. Hart, 2007, Fabrication and Characterisation of Novel Screen-Printed Tubular Microband Electrodes, and Their Application to the Measurement of Hydrogen Peroxide, Electrochim. Acta, 52, 7248-7253.

    [10] L. Authier, C. Grossiord, P. Brossier, B. Limoges, 2001, Gold Nanoparticle-Based Quantitative Electrochemical Detection of Amplified Human Cytomegalovirus DNA Using Disposable Microband Electrodes, Anal. Chem. 73, 4450-4456.

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