The immunological analysis of epitopes on hCG

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Lund, Torben ; Delves, Peter J. (1998)

The heterodimeric glycoprotein hormone, human chorionic gonadotropin, has been extensively characterized in terms of its recognition by mouse monoclonal antibodies. A number of different approaches have led to the definition of several epitope clusters on the surface of the molecule. These include epitopes located solely on the alpha- or beta-chain, some of which are masked when the two chains associate to form the holo-hormone. Additional epitopes comprise amino acids contributed by both the chains. In contrast to the extensive knowledge regarding B cell epitopes, the characterization of T cell epitopes on hCG has only recently begun to be explored.
  • References (33)
    33 references, page 1 of 4

    Acevedo HF, Tong JY and Hartsock RJ (1995) Human chorionic gonadotropinb subunit gene expression in cultured human fetal and cancer cells of different types and origins Cancer 76 1467-1475

    Berger P, Panmoung W, Khaschabi D, Mayregger B and Wick G (1988) Antigenic features of human follicle stimulating hormone delineated by monoclonal antibodies and construction of an immunoradiometric assay Endocrinology 123 2351-2359

    Berger P, Klieber R, Panmoung W, Madersbacher S, Wolf H and Wick G (1990) Monoclonal antibodies against the free subunits of human chorionic gonadotrophin Journal of Endocrinology 125 301-309

    Bidart JM, Ozturk M, Bellet DH, Jolivet M, Gras MH, Troalen F, Bohuon CJ and Wands J R (1985) Identification of epitopes associated with hCG and the b hCG carboxyl terminus by monoclonal antibodies produced against a synthetic peptide Journal of Immunology 134 457-464

    Bottger V, Micheel B, Scharte G, Kaiser G, Wolf G and Schmechta H (1993) Monoclonal antibodies to human chorionic gonadotropin (hCG) and their use in two-site binding enzyme immunoassays Hybridoma 12 81-91

    Cosowsky L, Rao SN, MacDonald GJ, Papkoff H, Campbell RK and Moyle WR (1995) The groove between the a - and b -subunits of hormones with lutropin (LH) activity appears to contact the LH receptor, and its conformation is changed during hormone binding Journal of Biological Chemistry 270 20 011-20 019

    Delves PJ, Lund T and Roitt IM (1997) Can epitope-focused vaccines select advantageous immune responses? Molecular Medicine Today 3 55-60

    Deshmukh US, Pal R, Talwar GP and Gupta SK (1993) Antibody response against epitopes on hCG mapped by monoclonal antibodies in women immunized with an anti-hCG vaccine and its implications for bioneutralization Journal of Reproductive Immunology 25 103-117

    Dirnhofer S, Klieber R, Deleeuw R, Bidart JM, Merz WE, Wick G and Berger P (1993) Functional and immunological relevance of the COOHterminal extension of human chorionic gonadotropin-b - implications for the WHO birth-control vaccine FASEB Journal 7 1381-1385

    Dirnhofer S, Lechner O, Madersbacher S, Klieber R, de Leeuw R, Wick G and Berger P (1994a) Free a subunit of human chorionic gonadotrophin: molecular basis of immunologically and biologically active domains Journal of Endocrinology 140 145-154

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