Poly(amidoamine)s synthesis, characterisation and interaction with BSA

Article English OPEN
Dubois, Julie L. N. ; Lavignac, Nathalie (2014)

Cationic poly(amidoamine)s (PAAs) were synthesised and characterised by NMR and gel permeation chromatography. Their thermal properties were investigated using thermogravimetric analysis and differential scanning calorimetry. Although poly(amidoamine)s have been used as endosomolytic polymers for protein intracellular delivery, the interaction of the polymers with the proteins still need to be investigated. BSA was used as a model protein and complexation with the different poly(amidoamine) s was investigated using gel retardation assays, fluorescence spectroscopy and high sensitivity differential scanning calorimetry. Our results indicate that the thermal stability of BSA was affected upon interaction and complexation with the poly(amidoamine)s, however these interactions did not seem to modify the structure of the protein. Polymer flexibility seemed to favour polymer/protein complexation and promoted thermal stability.
  • References (33)
    33 references, page 1 of 4

    1 V. Torchilin, Drug Discovery Today, 2008, 5, 95-103.

    2 B. Leader, Q. J. Baca and D. E. Golan, Nat. Rev. Drug Discovery, 2008, 7, 21-39.

    3 Z. Gu, A. Biswas, M. X. Zhao and Y. Tang, Chem. Soc. Rev., 2011, 40, 3638-3655.

    4 P. Ferruti, J. Polym. Sci., Part A: Polym. Chem., 2013, 51, 2319- 2353.

    5 S. C. W. Richardson, N. G. Pattrick, N. Lavignac, P. Ferruti and R. Duncan, J. Controlled Release, 2010, 142, 78-88.

    6 N. Lavignac, J. L. Nicholls, P. Ferruti and R. Duncan, Macromol. Biosci., 2009, 9, 480-487.

    7 E. Ranucci, P. Ferruti, E. Lattanzio, A. Manfredi, M. Rossi, P. A. Mussini, F. Chiellini and C. Bartoli, J. Polym. Sci., Part A: Polym. Chem., 2009, 47, 6977-6991.

    8 W. M. Liu, M. Liu, Y. N. Xue, N. Peng, X. M. Xia, R. X. Zhuo and S. W. Huang, J. Biomed. Mater. Res., 2012, 100, 872-881.

    9 L. J. van der Aa, P. Vader, G. Storm, R. M. Schiffelers and J. F. J. Engbersen, J. Controlled Release, 2011, 150, 177-186.

    10 P. Ferruti, S. Manzoni, S. C. W. Richardson, R. Duncan, N. G. Pattrick, R. Mendichi and M. Casolaro, Macromolecules, 2000, 33, 7793-7800.

  • Bioentities (1)
    4f5s Protein Data Bank
  • Metrics
    No metrics available
Share - Bookmark