Orchestrated transcription of biological processes in the marine picoeukaryote Ostreococcus exposed to light/dark cycles\ud

Article English OPEN
Monnier, Annabelle ; Liverani, Silvia ; Bouvet, Régis ; Jesson, Béline ; Smith, J. Q. ; Mosser, Jean ; Corellou, Florence ; Bouget, François-Yves (2010)

Background: Picoeukaryotes represent an important, yet poorly characterized component of marine phytoplankton. The recent genome availability for two species of Ostreococcus and Micromonas has led to the emergence of picophytoplankton comparative genomics. Sequencing has revealed many unexpected features about genome structure and led to several hypotheses on Ostreococcus biology and physiology. Despite the accumulation of genomic data, little is known about gene expression in eukaryotic picophytoplankton. \ud Results: We have conducted a genome-wide analysis of gene expression in Ostreococcus tauri cells exposed to light/dark cycles (L/D). A Bayesian Fourier Clustering method was implemented to cluster rhythmic genes according to their expression waveform. In a single L/D condition nearly all expressed genes displayed rhythmic patterns of expression. Clusters of genes were associated with the main biological processes such as transcription in the nucleus and the organelles, photosynthesis, DNA replication and mitosis. \ud Conclusions: Light/Dark time-dependent transcription of the genes involved in the main steps leading to protein synthesis (transcription basic machinery, ribosome biogenesis, translation and aminoacid synthesis) was observed, to an unprecedented extent in eukaryotes, suggesting a major input of transcriptional regulations in Ostreococcus. We propose that the diurnal co-regulation of genes involved in photoprotection, defence against oxidative stress and DNA repair might be an efficient mechanism, which protects cells against photo-damage thereby, contributing to the ability of O. tauri to grow under a wide range of light intensities.
  • References (35)
    35 references, page 1 of 4

    1. Not F, Latasa M, Marie D, Cariou T, Vaulot D, Simon N: A single species, Micromonas pusilla (Prasinophyceae), dominates the eukaryotic picoplankton in the Western English Channel. Appl Environ Microbiol 2004, 70:4064-4072.

    2. Viprey M, Guillou L, Ferreol M, Vaulot D: Wide genetic diversity of picoplanktonic green algae (Chloroplastida) in the Mediterranean Sea uncovered by a phylum-biased PCR approach. Environ Microbiol 2008, 10:1804-1822.

    3. Vaulot D, Eikrem W, Viprey M, Moreau H: The diversity of small eukaryotic phytoplankton (< or = 3 microm) in marine ecosystems. FEMS Microbiol Rev 2008, 32:795-820.

    4. Courties C, Vaquer A, Trousselier M, Lautier J, Chrétiennot-Dinet M-J, Neveux J, Machado C, Claustre H: Smallest eukarotic organism. Nature 1994, 370:255.

    5. Derelle E, Ferraz C, Rombauts S, Rouze P, Worden AZ, Robbens S, Partensky F, Degroeve S, Echeynie S, Cooke R, et al: Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features. Proc Natl Acad Sci USA 2006, 103:11647-11652.

    6. Palenik B, Grimwood J, Aerts A, Rouze P, Salamov A, Putnam N, Dupont C, Jorgensen R, Derelle E, Rombauts S, et al: The tiny eukaryote Ostreococcus provides genomic insights into the paradox of plankton speciation. Proc Natl Acad Sci USA 2007, 104:7705-7710.

    7. Worden AZ, Lee JH, Mock T, Rouze P, Simmons MP, Aerts AL, Allen AE, Cuvelier ML, Derelle E, Everett MV, et al: Green evolution and dynamic adaptations revealed by genomes of the marine picoeukaryotes Micromonas. Science 2009, 324:268-272.

    8. Six C, Worden AZ, Rodriguez F, Moreau H, Partensky F: New insights into the nature and phylogeny of prasinophyte antenna proteins: Ostreococcus tauri, a case study. Mol Biol Evol 2005, 22:2217-2230.

    9. Jancek S, Gourbiere S, Moreau H, Piganeau G: Clues about the genetic basis of adaptation emerge from comparing the proteomes of two Ostreococcus ecotypes (Chlorophyta, Prasinophyceae). Mol Biol Evol 2008, 25:2293-2300.

    10. Piganeau G, Vandepoele K, Gourbiere S, Peer Van de Y, Moreau H: Unravelling cis-regulatory elements in the genome of the smallest photosynthetic eukaryote: phylogenetic footprinting in Ostreococcus. J Mol Evol 2009, 69:249-259.

  • Related Research Results (1)
  • Metrics
    0
    views in OpenAIRE
    0
    views in local repository
    24
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    Warwick Research Archives Portal Repository - IRUS-UK 0 24
Share - Bookmark