Arbuscular mycorrhizal community composition associated with two plant species in a grassland ecosystem

Article English OPEN
Duck, J.M. ; Young, J.P.W. ; Vandenkoornhuyse, P. ; Husband, R. ; Daniell, T.J. ; Watson, I.J. ; Fitter, A.H. (2002)

Arbuscular mycorrhizal (AM) fungi are biotrophic symbionts colonizing about two-thirds of land plant species and found in all ecosystems. They are of major importance in plant nutrient supply and their diversity is suggested to be an important determinant of plant community composition. The diversity of the AM fungal community composition in the roots of two plant species (Agrostis capillaris and Trifolium repens) that co-occurred in the same grassland ecosystem was characterized using molecular techniques. We analysed the small subunit (SSU) ribosomal RNA gene amplified from a total root DNA extract using AM fungal-specific primers. A total of 2001 cloned fragments from 47 root samples obtained on four dates were analysed by restriction fragment length polymorphism, and 121 of them were sequenced. The diversity found was high: a total of 24 different phylotypes (groups of phylogenetically related sequences) colonized the roots of the two host species. Phylogenetic analyses demonstrate that 19 of these phylotypes belonged to the Glomaceae, three to the Acaulosporaceae and two to the Gigasporaceae. Our study reveals clearly that the AM fungal community colonizing T. repens differed from that colonizing A. capillaris, providing evidence for AM fungal host preference. In addition, our results reveal dynamic changes in the AM fungal community through time.
  • References (44)
    44 references, page 1 of 5

    Bever JD (1999) Dynamics within mutualism and the maintenance of diversity: inference from a model of interguild frequency dependence. Ecology Letters, 2, 52 - 61.

    Bever JD, Morton JB, Antonovics J, Schultz PA (1996) Hostdependent sporulation and species diversity of arbuscular mycorrhizal fungi in a mown grassland. Journal of Ecology, 84, 71 - 82.

    Cavalier-Smith T, Allsopp MTEP (1996) Corallochytrium, an enigmatic non-flagellate protozoan related to choanoflagellates. European Journal of Protistology, 32, 306 - 310.

    Clapp JP, Fitter AH, Young JPW (1999) Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp. Molecular Ecology, 8, 915 - 921.

    Clapp JP, Helgason T, Daniell TJ, Young JPW (2002) Genetic studies of the structure and diversity of arbuscular mycorrhizal fungal communities. In: Mycorrhizal Ecology (eds van der Heijden MGA, Sanders IR), pp. 201-224. Springer, Heidelberg.

    Daniell TJ, Husband R, Fitter AH, Young JPW (2001) Molecular diversity of arbuscular mycorrhizal fungi colonising arable crops. FEMS Microbiology Ecology, 36, 203 - 209.

    Del Val C, Barea JM, Azcón-Aguilar C (1999) Diversity of arbuscular mycorrhizal fungus populations in heavy-metal-contaminated soils. Applied and Environmental Microbiology, 65, 718 - 723.

    Edwards SG, Fitter AH, Young JPW (1997) Quantification of an arbuscular mycorrhizal fungus, Glomus mosseae, within plant roots by competitive polymerase chain reaction. Mycological Research, 101, 1440 - 1444.

    Eom A-H, Hartnett DC, Wilson GWT (2000) Host plant species effects on arbuscular mycorrhizal fungal communities in tallgrass prairie. Oecologia, 122, 435 - 444.

    Gehrig H, Schüßler A, Kluge M (1996) Geosiphon pyriforme, a fungus forming endocytobiosis with Nostoc (cyanobacteria), is an ancestral member of the Glomales: evidence by SSU rRNA analysis. Journal of Molecular Evolution, 43, 71 - 81.

  • Metrics
    0
    views in OpenAIRE
    0
    views in local repository
    190
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    White Rose Research Online - IRUS-UK 0 190
Share - Bookmark