Overexpression of beta-carotene hydroxylase enhances stress tolerance in Arabidopsis

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Davison, P.A. ; Hunter, C.N. ; Horton, P. (2002)
  • Subject:
    mesheuropmc: food and beverages | fungi

Plant stress caused by extreme environmental conditions is already a principal reason for yield reduction in crops. The threat of global environment change makes it increasingly important to generate crop plants that will withstand such conditions. Stress, particularly stress caused by increased sunlight, leads to the production of reactive oxygen species that cause photo-oxidative cell damage. Carotenoids, which are present in the membranes of all photosynthetic organisms, help protect against such light-dependent oxidative damage. In plants, the xanthophyll cycle (the reversible interconversion of two carotenoids, violaxanthin and zeaxanthin) has a key photoprotective role and is therefore a promising target for genetic engineering to enhance stress tolerance. Here we show that in Arabidopsis thaliana overexpression of the chyB gene that encodes -carotene hydroxylase—an enzyme in the zeaxanthin biosynthetic pathway—causes a specific twofold increase in the size of the xanthophyll cycle pool. The plants are more tolerant to conditions of high light and high temperature, as shown by reduced leaf necrosis, reduced production of the stress indicator anthocyanin and reduced lipid peroxidation. Stress protection is probably due to the function of zeaxanthin in preventing oxidative damage of membranes.
  • References (35)
    35 references, page 1 of 4

    15. Oldenborg, P. A. et al. Role of CD47 as a marker of self on red blood cells. Science 288, 2051-2054 (2000).

    16. Mansfield, P. J., Boxer, L. A. & Suchard, S. J. Thrombospondin stimulates motility of human neutrophils. J. Cell Biol. 111, 3077-3086 (1990).

    17. Savill, J. S. et al. C. Macrophage phagocytosis of aging neutrophils in inflammation. Programmed cell death in the neutrophil leads to its recognition by macrophages. J. Clin. Invest. 83, 865-875 (1989).

    18. Brown, S. B., Bailey, K. & Savill, J. Actin is cleaved during constitutive apoptosis. Biochem. J. 323, 233-237 (1997).

    19. Bird, I. N. et al. Homophilic PECAM-1 (CD31) interactions prevent endothelial cell apoptosis but do not support cell spreading or migration. J. Cell Sci. 112, 1989-1997 (1999).

    20. Brown, S. B., Clarke, M. C., Magowan, L., Sanderson, H. & Savill, J. Constitutive death of platelets leading to scavenger receptor-mediated phagocytosis. A caspase-independent cell clearance program. J. Biol. Chem. 275, 5987-5996 (2000).

    21. Metzelaar, M. J., Korteweg, J., Sixma, J. J. & Nieuwenhuis, H. K. Biochemical characterization of PECAM-1 (CD31 antigen) on human platelets. Thromb. Haemost. 66, 700-707 (1991).

    3. Cogdell, R. J. & Frank, H. A. How carotenoids function in photosynthetic bacteria. Biochim. Biophys. Acta 895, 63-79 (1987).

    4. Yamamoto, H. Y., Nakayama, T. O. M. & Chichester, C. O. Studies on the light and dark interconversions of leaf xanthophylls. Arch. Biochem. Biophys. 97, 168-173 (1962).

    5. Demmig-Adams, B. & Adams, W. W. Photoprotection and other responses of plants to high light stress. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43, 599-626 (1992).

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