Identification of pathogenicity-related genes in Fusarium oxysporum f. sp. cepae

Article English OPEN
Taylor, Andrew ; Vágány, Viktória ; Jackson, Alison C. ; Harrison, Richard J. ; Rainoni, Alessandro ; Clarkson, John P. (2016)
  • Publisher: Wiley-Blackwell Publishing Ltd.
  • Journal: Molecular Plant Pathology, volume 17, issue 7, pages 1,032-1,047 (issn: 1464-6722, eissn: 1364-3703)
  • Related identifiers: pmc: PMC4982077, doi: 10.1111/mpp.12346, doi: 10.1111/mpp.12346
  • Subject: Fusarium oxysporum f. sp. cepae | QR | Fusarium basal rot | onion | effector genes | SB | Original Article | pathogenicity | Original Articles | QK | S1 | Secreted In Xylem (SIX)
    mesheuropmc: food and beverages

Pathogenic isolates of Fusarium oxysporum, distinguished as formae speciales (f. spp.) based on their host specificity, cause crown rots, root rots and vascular wilts on many important crops worldwide. F. oxysporum f. sp. cepae (FOC) is particularly problematic to onion growers worldwide and is increasing in prevalence in the UK. We characterised 31 F. oxysporum isolates collected from UK onions using pathogenicity tests, sequencing of housekeeping genes and identification of effectors. In onion seedling and bulb tests, 21 isolates were pathogenic while 10 were non-pathogenic. Molecular characterisation of these isolates, and 21 additional isolates comprising other f. spp. and different Fusarium species, was carried out by sequencing three housekeeping genes. A concatenated tree separated the F. oxysporum isolates into six clades but did not distinguish pathogenic and non-pathogenic isolates. Ten putative effectors were identified within FOC, including seven Secreted in Xylem (SIX) genes first reported in F. oxysporum f. sp. lycopersici. Two highly homologous proteins with signal peptides and RxLR motifs (CRX1/CRX2) and a gene with no previously characterised domains (C5) were also identified. The presence/absence of nine of these genes was strongly related to pathogenicity against onion and all were shown to be expressed in planta. Different SIX gene complements were identified in other f. spp. but none were identified in three other Fusarium species from onion. Although the FOC SIX genes had a high level of homology with other f. spp. there were clear differences in sequence which were unique to FOC while CRX1 and C5 genes appear to be largely FOC specific. This article is protected by copyright. All rights reserved.
  • References (64)
    64 references, page 1 of 7

    Bayraktar, H., Tu€rkkan, M. and Dolar, F.S. (2010) Characterization of Fusarium oxysporum f.sp. cepae from onion in Turkey based on vegetative compatibility and rDNA RFLP analysis. J. Phytopathol. 158, 691-697.

    Boratyn, G.M., Camacho, C., Cooper, P.S., Coulouris, G., Fong, A., Ma, N., Madden, T.L., Matten, W.T., McGinnis, S.D., Merezhuk, Y., Raytselis, Y., Sayers, E.W., Tao, T., Ye, J. and Zaretskaya, I. (2013) BLAST: a more efficient report with usability improvements. Nucleic Acids Res. 41, W29-W33.

    Brayford, D. (1996) IMI descriptions of fungi and bacteria set 127. Mycopathologia, 133, 35-63.

    Broad Institute/MIT. (2007) Fusarium Comparative Sequencing Project. Available at: http://www.broadinstitute.org/. Accessed March 2015.

    Chakrabarti, A., Rep, M., Wang, B., Ashton, A., Dodds, P. and Ellis, J. (2011) Variation in potential effector genes distinguishing Australian and non-Australian isolates of the cotton wilt pathogen Fusarium oxysporum f.sp. vasinfectum. Plant Pathol. 60, 232-243.

    Covey, P.A., Kuwitzky, B., Hanson, M. and Webb, K.M. (2014) Multilocus analysis using putative fungal effectors to describe a population of Fusarium oxysporum from sugar beet. Phytopathology, 104, 886-896.

    Cramer, C. (2000) Breeding and genetics of Fusarium basal rot resistance in onion. Euphytica, 115, 159-166.

    Dean, R., Van Kan, J.A., Pretorius, Z.A., Hammond-Kosack, K.E., Di Pietro, A., Spanu, P.D., Rudd, J.J., Dickman, M., Kahmann, R., Ellis, J. and Foster, G.D. (2012) The Top 10 fungal pathogens in molecular plant pathology. Mol. Plant Pathol. 13, 414-430.

    Dissanayake, M., Kashima, R., Tanaka, S. and Ito, S.-I. (2009a) Genetic diversity and pathogenicity of Fusarium oxysporum isolated from wilted Welsh onion in Japan. J. Gen. Plant Pathol. 75, 125-130.

    Dissanayake, M., Kashima, R., Tanaka, S. and Ito, S.-I. (2009b) Pathogenic variation and molecular characterization of Fusarium species isolated from wilted Welsh onion in Japan. J. Gen. Plant Pathol. 75, 37-45.

  • Metrics
    No metrics available