A high-throughput screening method for determining the substrate scope of nitrilases

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Black, Gary ; Brown, Nicola ; Perry, Justin ; Randall, P. David ; Turnbull, Graeme ; Zhang, Meng (2015)

Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.
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