Genetic expression of an amyloid peptide fragment and analysis of formylated products

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Cheng, Ge ; Krasel, Cornelius ; Zhou, H. G. ; Chappell, David ; Hamley, Ian W. (2011)

The model amyloid peptide AAKLVFF was expressed as a His-tagged fusion protein with the immunoglobulin-binding domain B1 of streptococcal\ud protein G (GB1), a small (56 residues), stable, single-domain protein. It is shown that expression of this model amyloid peptide is possible and is\ud not hindered by aggregation. Formylation side reactions during the CNBr cleavage are investigated via synthesis of selectively formylated peptides.
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