Arginine-glycine-aspartic acid functional branched semi-interpenetrating hydrogels.

Article English OPEN
Plenderleith, R.A. ; Pateman, C.J. ; Rodenburg, C. ; Haycock, J.W. ; Claeyssens, F. ; Sammon, C. ; Rimmer, S. (2015)
  • Publisher: Royal Society of Chemistry
  • Related identifiers: doi: 10.1039/C5SM00695C
  • Subject:
    mesheuropmc: macromolecular substances | technology, industry, and agriculture

For the first time a series of functional hydrogels based on semi-interpenetrating networks with both\ud branched and crosslinked polymer components have been prepared and we show the successful use of\ud these materials as substrates for cell culture. The materials consist of highly branched poly(N-isopropyl\ud acrylamide)s with peptide functionalised end groups in a continuous phase of crosslinked poly(vinyl\ud pyrrolidone). Functionalisation of the end groups of the branched polymer component with the GRGDS\ud peptide produces a hydrogel that supports cell adhesion and proliferation. The materials provide a new\ud synthetic functional biomaterial that has many of the features of extracellular matrix, and as such can be\ud used to support tissue regeneration and cell culture. This class of high water content hydrogel material\ud has important advantages over other functional hydrogels in its synthesis and does not require postprocessing\ud modifications nor are functional-monomers, which change the polymerisation process,\ud required. Thus, the systems are amenable to large scale and bespoke manufacturing using conventional\ud moulding or additive manufacturing techniques. Processing using additive manufacturing is exemplified\ud by producing tubes using microstereolithography.
  • References (41)
    41 references, page 1 of 5

    and (iii) a computer controlled z-axis translation stage (Thorlabs 19 S. Rimmer, S. Carter, R. Rutkaite, J. W. Haycock and

    Ltd, Cambridgeshire, UK). A color inverted image was created L. Swanson, Soft Matter, 2007, 3, 971-973.

    in MS paint and uploaded to the DMD by control software 20 Y. Guan and Y. Zhang, Soft Matter, 2011, 7, 6375-6384.

    (ALP basic). The laser power was set to 10 mV and projected via 21 Z. M. O. Rzaev, S. Dinçer and E. Pis-kin, Prog. Polym. Sci.,

    a beam expander onto the DMD. The image from the DMD 2007, 32, 534-595.

    was focused via several lenses onto the surface of the monomer. 22 B. R. Saunders, N. Laajam, E. Daly, S. Teow, X. Hu and

    The z-stage was moved in a downwards direction at a speed of R. Stepto, Adv. Colloid Interface Sci., 2009, 147-148, 251-262.

    0.03 mm s 1 allowing the polymerisation to occur at the surface 23 Q. Yu, L. M. Johnson and G. P. Lo´pez, Adv. Funct. Mater.,

    of the monomer. 2014, 24, 3751-3759. 24 Y. Morita and I. Kaetsu, Radiat. Phys. Chem., 1992, 39, 473-476. 25 D. Schmaljohann, Adv. Drug Delivery Rev., 2006, 58, 1655-1670.

    Acknowledgements 26 T. Owaki, T. Shimizu, M. Yamato and T. Okano, Biotechnol. J., 2014, 9, 904-914.

  • Metrics
    No metrics available
Share - Bookmark