A Novel Method for Isolation and Recovery of Ceramic Nanoparticles and Metal Wear Debris from Serum Lubricants at Ultra-low Wear Rates

Article English OPEN
Lal, S ; Hall, RM ; Tipper, JL (2016)
  • Publisher: Elsevier

Ceramics have been used to deliver significant improvements in the wear properties of orthopaedic bearing materials, which has made it challenging to isolate wear debris from simulator lubricants. Ceramics such as silicon nitride, as well as ceramic-like surface coatings on metal substrates have been explored as potential alternatives to conventional implant materials. Current isolation methods were designed for isolating conventional metal, UHMWPE and ceramic wear debris. In this paper, we describe a methodology for isolation and recovery of ceramic or ceramic-like coating particles and metal wear particles from serum lubricants under ultra-low and low wear performance. Enzymatic digestion was used to digest the serum proteins and sodium polytungstate was used as a novel density gradient medium to isolate particles from proteins and other contaminants by ultracentrifugation. This method demonstrated over 80% recovery of particles and did not alter the size or morphology of ceramic and metal particles during the isolation process.
  • References (43)
    43 references, page 1 of 5

    [1] [2] [3] Z.M. Jin, J. Fisher, Tribology in joint replacement, in: P.A. Revell (Ed.), Jt. Replace. Technol., 2nd ed., Woodhead Publishing, 2008, pp. 47 - 51.

    T.R. Green, J. Fisher, M. Stone, B.M. Wroblewski, E. Ingham, Polyethylene particles of a “critical size” are necessary for the induction of cytokines by macrophages in vitro., Biomaterials. 19 (1998) 2297- 302.

    J.B. Matthews, T.R. Green, M.H. Stone, B.M. Wroblewski, J. Fisher, E. Ingham, Comparison of the response of primary human peripheral blood mononuclear phagocytes from different donors to challenge with model polyethylene particles of known size and dose., Biomaterials. 21 (2000) 2033- [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] T.R. Green, J. Fisher, J.B. Matthews, M.H. Stone, E. Ingham, Effect of size and dose on bone resorption activity of macrophages by in vitro clinically relevant ultra high molecular weight polyethylene particles., J. Biomed. Mater. Res. 53 (2000) 490-7.

    R.L. Illgen, T.M. Forsythe, J.W. Pike, M.P. Laurent, C.R. Blanchard, Highly Crosslinked vs Conventional Polyethylene Particles-An In Vitro Comparison of Biologic Activities, J. Arthroplasty. 23 (2008) 721- 731.

    Bone Joint Surg. Am. 82 (2000) 457-76.

    A.P.D. Elfick, S.M. Green, S. Krikler, A. Unsworth, The nature and dissemination of UHMWPE wear debris retrieved from periprosthetic tissue of THR., J. Biomed. Mater. Res. A. 65 (2003) 95-108.

    C.P. Case, V.G. Langkamer, C. James, M.R. Palmer, A.J. Kemp, P.F. Heap, L. Solomon, Widespread dissemination of metal debris from implants., J. Bone Joint Surg. Br. 76 (1994) 701-12.

    N. Doshi, S. Mitragotri, Macrophages recognize size and shape of their targets., PLoS One. 5 (2010) e10051.

    H. Yue, W. Wei, Z. Yue, P. Lv, L. Wang, G. Ma, Z. Su, Particle size affects the cellular response in macrophages, Eur. J. Pharm. Sci. 41 (2010) 650-657.

    F. Billi, P. Benya, A. Kavanaugh, J. Adams, H. McKellop, E. Ebramzadeh, The John Charnley Award: an accurate and extremely sensitive method to separate, display, and characterize wear debris: part 2: metal and ceramic particles., Clin. Orthop. Relat. Res. 470 (2012) 339-50.

  • Related Research Results (1)
  • Similar Research Results (1)
  • Metrics
    0
    views in OpenAIRE
    0
    views in local repository
    40
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    White Rose Research Online - IRUS-UK 0 40
Share - Bookmark