Towards pain-free diagnosis of skin diseases through\ud multiplexed microneedles: biomarker extraction and\ud detection using a highly sensitive blotting method

Article English OPEN
Ng, Keng Wooi ; Lau, Wing Man ; Williams, Adrian C. (2015)

Immunodiagnostic microneedles provide a novel way to extract protein biomarkers from the skin in a minimally invasive manner for analysis in vitro. The technology could overcome challenges in biomarker analysis specifically in solid tissue, which currently often involves invasive biopsies. This study describes the development of a multiplex immunodiagnostic device incorporating mechanisms to detect multiple antigens simultaneously, as well as internal assay controls for result validation. A novel detection method is also proposed. It enables signal detection specifically at microneedle tips and therefore may aid the construction of depth profiles of skin biomarkers. The detection method can be coupled with computerised densitometry for signal quantitation. The antigen specificity, sensitivity and functional stability of the device were assessed against a number of model biomarkers. Detection and analysis of endogenous antigens (interleukins 1α and 6) from the skin using the device was demonstrated. The results were verified using conventional enzyme-linked immunosorbent assays. The detection limit of the microneedle device, at ≤10 pg/mL, was at least comparable to conventional plate-based solid-phase enzyme immunoassays.
  • References (26)
    26 references, page 1 of 3

    [1] Gremel G, Grannas K, Sutton LA, Pontén F, Zieba A (2013) In situ Protein Detection for Companion Diagnostics. Front Oncol 3:271.

    [5] Skehel JM (2004) Preparation of extracts from animal tissues. In: Cutler P (ed) Protein Purification Protocols, 2nd ed. Humana Press, Totowa, NJ, pp 15-20

    [6] Kim Y-C, Park J-H, Prausnitz MR (2012) Microneedles for drug and vaccine delivery. Adv Drug Deliv Rev 64:1547-1568.

    [7] Tuan-Mahmood T-M, McCrudden MTC, Torrisi BM, McAlister E, Garland MJ, Singh TRR, Donnelly RF (2013) Microneedles for intradermal and transdermal drug delivery. Eur J Pharm Sci 50:623-637.

    [8] Gill HS, Denson DD, Burris BA, Prausnitz MR (2008) Effect of microneedle design on pain in human volunteers. Clin J Pain 24:585-594.

    [9] Haq MI, Smith E, John DN, Kalavala M, Edwards C, Anstey A, Morrissey A, Birchall JC (2009) Clinical administration of microneedles: skin puncture, pain and sensation. Biomed Microdevices 11:35-47.

    [10] Bhargav A, Muller DA, Kendall MAF, Corrie SR (2012) Surface modifications of microprojection arrays for improved biomarker capture in the skin of live mice. ACS Appl Mater Interfaces 4:2483-2489.

    [11] Coffey JW, Corrie SR, Kendall MAF (2013) Early circulating biomarker detection using a wearable microprojection array skin patch. Biomaterials 34:9572-9583.

    [12] Lee KT, Muller DA, Coffey JW, Robinson KJ, McCarthy JS, Kendall MAF, Corrie SR (2014) Capture of the Circulating Plasmodium falciparum Biomarker HRP2 in a Multiplexed Format, via a Wearable Skin Patch. Anal Chem 86:10474-10483.

    [13] Yeow B, Coffey JW, Muller DA, Grøndahl L, Kendall MAF, Corrie SR (2013) Surface modification and characterization of polycarbonate microdevices for capture of circulating biomarkers, both in vitro and in vivo. Anal Chem 85:10196-10204.

  • Metrics
    0
    views in OpenAIRE
    0
    views in local repository
    109
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    Central Archive at the University of Reading - IRUS-UK 0 109
Share - Bookmark