Ultrafiltration based purification strategies for surfactin produced by bacillus subtilis lb5a using cassava wastewater as substrate

Article English OPEN
De Andrade, Cristiano J. ; Barros, Francisco F. C. ; De Andrade, Lidiane M. ; Roco, Silvana A. ; Sforca, Mauricio L. ; Pastore, Glaucia M. ; Jauregi, Paula (2016)

BACKGROUND: Bacillus subtilis synthesizes surfactin, a powerful surface-active agent. It has interesting potential applications. However, due to its high cost of production, commercial use is impracticable. The downstream processing represents ≈60% of production costs and the culture medium ≈30%. Many reports focused, separately, on production of surfactin using by-products (reduced cost) or the purification using synthetic medium. Therefore, the aim of this work was to evaluate, for the first time, the impact of using a by-product as fermentation medium on the downstream processing based on membrane filtration.\ud RESULTS: Membranes of PES-100-kDa efficiently retained surfactin micelles - the first step of ultrafiltration, whereas, the second step required membranes of 50-kDa to separate surfactin monomers from proteins. Ultrafiltration of crude biosurfactant was associated with fouling and/or concentration polarization resulting in lower purity than when synthetic medium was used. Further improvement in purity was achieved by partial removal of proteins prior to ultrafiltration by precipitation and extraction. The RMN and MALDI-TOFMS analyses identified 11 potential surfactin homologous composed by two amino acid sequences.\ud CONCLUSION: Production of surfactin using cassava wastewater as a low-cost culture medium and its purification by the 2-step ultrafiltration process is feasible, nevertheless, the higher protein content of this medium as compared to the synthetic one leads to a lower purity product; further increase in purity can be achieved by applying additional purification steps prior to ultrafiltration with the subsequent increased in process cost.
  • References (24)
    24 references, page 1 of 3

    1. Barros FFC, Ponesi AN and Pastore GM, Production of biosurfactant by Bacillus subtilis LB5a on a pilot scale using cassava wastewater as substrate. J Ind Microbiol Biotechnol 35:1071-1078 (2008). DOI: 10.1007/s10295-008-0385-y

    2. Makkar RS, Cameotra SS and Banat IM, Advances in utilization of renewable substrates for biosurfactant production. AMB Express 1:1-19 (2011). DOI: 10.1186/2191-0855-1-5

    3. Pacwa-Płociniczak M, Płaza GA, Piotrowska-Seget Z and Cameotra SS, Environmental applications of biosurfactants: Recent Advances. Int J Mol Sci 12:633-654. (2011). DOI: 10.3390/ijms12010633

    4. Chen HL, Chen YS and Juang, RS, Recovery of surfactin from fermentation broths by a hybrid salting-out and membrane filtration process. Sep Purif Technol 59:244-252. (2008). DOI: 10.1016/j.seppur.2007.06.010

    5. Saharan BS, Sahu RK and Sharma D, A review on biosurfactants: fermentation, current developments and perspectives. Genet Eng Biotechnol J 2011:1-14. (2012).

    6. Jauregi P, Coutte F, Catiau L, Lecouturier D and Jacques P, Micelle size characterization of lipopeptides produced by B. subtilis and their recovery by the two-step ultrafiltration process Sep Purif Technol 104:175-182. (2013). DOI:10.1016/j.seppur.2012.11.017

    7. Chen HL, Chen YS and Juang, RS, Separation of surfactin from fermentation broths by acid precipitation and two-stage dead-end ultrafiltration processes. J Membr Sci 299:114-121. (2007). DOI: 10.1016/j.memsci.2007.04.031

    8. Chen HL, Chen YS and Juang RS, Flux decline and membrane cleaning in cross-flow ultrafiltration of treated fermentation broths for surfactin recovery. Sep Purif Technol 62:47- 55. (2008). DOI:10.1016/j.seppur.2007.12.015

    9. Isa MHM, Coraglia DE, Frazier RA and Jauregi P, Recovery and purification of surfactin from fermentation broth by a two step ultrafiltration process. J Membr Sci 296:51-57. (2007). DOI:10.1016/j.memsci.2007.03.023

    10. Isa MHM, Frazier RA and Jauregi P. A further study of the recovery and purification of surfactin from fermentation broth by membrane filtration. Sep Purif Technol 64:176-182. (2008). DOI:10.1016/j.seppur.2008.09.008

  • Metrics
    views in OpenAIRE
    views in local repository
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    Central Archive at the University of Reading - IRUS-UK 0 50
Share - Bookmark